Bordetella pertussis
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA35055 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Other | ||||
| DRR015887_1.fastq.gz | Fastqsanger.gz | |||
| DRR015887_2.fastq.gz | Fastqsanger.gz | |||
| DRR015965_1.fastq.gz | Fastqsanger.gz | |||
| DRR015965_2.fastq.gz | Fastqsanger.gz |
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Project description:To our knowledge, we provide the first proteomics study of Bordetella parapertussis, one of the causative agents of whooping cough. We compared the identified proteins different to Bordetella pertussis, the other pathogen causing whooping cough. In addition, we extended the study to investigate the proteome response to iron limitation, a stress condition the pathogens face while infection.
2020-02-05 | PXD012309 | Pride
Project description:Bordetella pertussis is the etiological agent of whooping cough, a bacterial infection of especially children, which may be fatal without treatment. In frame of studies to investigate putative effects of vaccination on host-pathogen interaction and clonal distribution of strains, in addition to Corynebacterium diphtheriae and Clostridium tetani toxoid vaccines, also whole-cell and acellular pertussis vaccines were analyzed by mass spectrometry.
2019-05-14 | PXD013804 | Pride
Project description:Copper is both essential and toxic to living beings, which therefore tightly control its intracellular concentration. At the host-pathogen interface, copper is used by phagocytic cells to kill invading microorganisms. We investigated copper homeostasis in the whooping cough agent Bordetella pertussis, which lives in the human respiratory mucosa and has no environmental reservoir. B. pertussis has considerably streamlined copper homeostasis mechanisms relative to other Gram-negative bacteria. Its single remaining defense line against copper intoxication consists in a metallochaperone diverted for copper passivation and two enzymes involved in peroxide detoxification, which together fight two stresses encountered in phagolysosomes. The three proteins are encoded by an original, composite operon assembled in an environmental ancestor and which is under sensitive control by copper. Interestingly, this system appears to play a role in persistent infection in the nasal cavity of B. pertussis-infected mice. Combining responses to co-occurring stresses in a tailored operon reveals a new strategy adopted by a host-restricted pathogen to optimize survival at minimal energy expenditure.
2020-10-27 | GSE145049 | GEO
Project description:Outbreaks of Bordetella pertussis (BP), the causative agent of whooping cough, continue despite broad vaccination coverage and have been increasing since vaccination switched from whole-BP (wP) to acellular BP (aP) vaccines. wP vaccination has been associated with more durable protective immunity and an induced Th1 polarized memory T cell response. Here, a multi-omics approach was applied to profile the immune response of 30 wP and 31 aP-primed individuals and identify correlates of T cell polarization before and after Tdap booster vaccination. We found that transcriptional changes indicating an interferon response on day 1 post-booster along with elevated plasma concentrations of IFN-γ and interferon-induced chemokines that peaked at day 1-3 post-booster correlated best with the Th1 polarization of the vaccine-induced memory T cell response on day 28. Our studies suggest that wP-primed individuals maintain their Th1 polarization through this early memory interferon response. This suggests that stimulating the interferon pathway during vaccination could be an effective strategy to elicit a predominant Th1 response in aP-primed individuals that protects better against infection.
2025-03-07 | GSE274944 | GEO
Project description:Comparative genomics of Bordetella pertussis isolates from children with whooping cough
| PRJEB42654 | ENA
Project description:Bordetella pertussis is a Gram-negative, strictly human respiratory pathogen and the causative agent of whooping cough (pertussis). Similar to other Gram-negative pathogens, B. pertussis produces a functional type III secretion system, but its role in pathogenesis of B. pertussis is enigmatic and has not yet been elucidated. Here, we applied omics RNA-seq as well as LC-MS/MS techniques and co-immunoprecipitation method to identify and characterize the novel CesT family T3SS chaperone BP2265. Our results show that the chaperone BP2265 specifically interacts with the secreted T3SS anti-sigma factor BtrA. Moreover, in the absence of the chaperone, secretion but not production of BtrA and several early, intermediate, and late T3SS substrates is severely impaired. It appears that the role of BtrA in regulating T3SS is more complex and extends beyond its activity as an antagonist of the sigma factor BtrS. We propose to rename BP2265 as BtcB for the Bordetella type III chaperone of BtrA.
2024-01-26 | PXD044487 | Pride
Project description:Bordetella pertussis is the bacterial causative agent of whooping cough, a serious respiratory illness. An extensive knowledge on its virulence regulation and metabolism is a key factor to ensure pertussis vaccine manufacturing process robustness. The aim of this study was to refine our comprehension of B. pertussis physiology along the fermentation process. A longitudinal multi-omics analysis was carried out over a 26-hour small-scale fermentation of B. pertussis. Fermentations were performed in batch mode and under culture conditions intending to mimic industrial processes. Putative cysteine and proline starvations were respectively observed at the beginning of the exponential phase (from 4h to 8h) and during the exponential phase (18h45). As revealed by multi-omics analyses, the proline starvation induced major molecular changes, including a transient metabolism with internal stock consumption. In the meantime, growth and specific total PT, PRN and Fim2 antigen productions were negatively affected. Interestingly, the master virulence-regulating two-component system of B. pertussis (BvgASR) was not evidenced as the sole virulence regulator in this in vitro growth condition. Indeed, novel intermediate regulators were identified as putatively involved in the expression of some virulence-activated genes (vags). Such longitudinal multi-omics analysis applied to B. pertussis fermentation process emerges as a powerful tool for characterization and incremental optimization of vaccine antigen production.
2023-03-11 | PXD039615 | Pride
Project description:Bordetella pertussis, the causative agent of whooping cough, produces a microcapsule at its surface but its role in pertussis pathogenesis remained to be investigated.Absence of KpsT, a membrane-associated protein involved in the polysaccharide transport resulted in the down-modulation of a large number of virulence genes which correlated with strong attenuation in vivo.
2014-10-07 | GSE62088 | GEO
Project description:Acetylation on ε-amino groups of lysine residues (N-ε-lysine acetylation) represents an important mechanism of post-translational regulation of protein function. However, its role and extent in the whooping cough agent Bordetella pertussis remain unknown. In this study, we analyzed the acetylomes of two bacterial mutants lacking putative lysine deacetylases encoded by genes BP0960 and BP3063 and compared them with the acetylome of wild-type B. pertussis. The results suggest that acetylation on lysine residues may modulate the activities of proteins involved in bacterial virulence and of multiple histone-like proteins.
2020-07-19 | PXD016384 | Pride
Project description:Bordetella pertussis, the etiological agent of whooping cough, regulates the expression of its virulence factors by the well-defined BvgA/S two-component regulatory system. Phosphorylated BvgA activates the virulence activated genes (vags) and represses via the activation of the bvgR gene the expression of the virulence repressed genes (vrgs). In the presence of MgSO4, known as modulating condition, the BvgA/S system is inactive, and the vrgs are expressed. Here, we show that, except for 6 genes of unknown function, the expression of all the vrgs depends on RisA, another transcriptional two-component regulator. In addition to the vrgs, RisA also regulates the expression of a many other genes. Whereas the expression of most vags was not affected by the deletion of risA, some vags, including the genes coding for pertactin, filamentous hemagglutinin, fimbriae and adenylate cyclase toxin, were surprisingly no longer modulated by MgSO4 in the risA- background. Yet another set of genes, mostly of unknown function, were modulated only in the risA- background. Finally, a large group of genes were up-regulated under modulating conditions only in the risA- background. They comprise genes of the chemotaxis and flagellar operons, iron-regulated genes, some of which are under the control of BvgA/S, while others are not. We identified RisK as the cognate RisA kinase, and found that most of the RisA-dependent genes also required RisK for expression, whereas a few genes were independent of RisK. These results were confirmed by the replacement of RisA with the phosphoablative RisAD60N analog and the phosphomimetic RisAD60E analog. Thus, according to its phosphorylation state, RisA regulates the expression of many genes, which may or may not also be regulated by the BvgA/S system, adding a new layer of complexity of B. pertussis gene regulation.
2016-08-20 | GSE77754 | GEO