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Mus musculus

ABSTRACT: PRC2 facilitates the regulatory topology required for poised enhancer function during pluripotent stem cell differentiation [4C-seq]

PROVIDER: PRJNA350733 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR4451730.fastq.gz	Fastqsanger.gz
	SRR4451731.fastq.gz	Fastqsanger.gz
	SRR4451732.fastq.gz	Fastqsanger.gz
	SRR4451723.fastq.gz	Fastqsanger.gz
	SRR4451733.fastq.gz	Fastqsanger.gz

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PRC2 facilitates the regulatory topology required for poised enhancer function during pluripotent stem cell differentiation

Project description:PRC2 facilitates the regulatory topology required for poised enhancer function during pluripotent stem cell differentiation

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Project description:PRC2 facilitates the regulatory topology required for poised enhancer function during pluripotent stem cell differentiation [RNA-seq EED]

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Poised enhancers regulatory activity is topologically facilitated by polycomb [RNA-seq LHX5]

Project description:Poised enhancers marked by H3K27me3 in pluripotent cells were previously proposed to facilitate the establishment of somatic expression programs upon embryonic stem cell (ESC) differentiation. However, the functional relevance and mechanism of action of poised enhancers remain unknown. Here, we use genetic deletions to demonstrate that poised enhancers are necessary for the induction of major anterior neural regulators. Mechanistically, poised enhancers enable RNA Polymerase II recruitment to their cognate promoters upon differentiation. Interestingly, poised enhancers already establish physical interactions with their target genes in ESC in a Polycomb repressive complex 2 (PRC2) dependent manner. Loss of PRC2 led to neither the activation of poised enhancers nor the induction of their putative target genes in undifferentiated ESC. In contrast, loss of PRC2 severely and specifically compromised the induction of major anterior neural genes representing poised enhancer targets. Overall, our work illuminates a novel function for polycomb proteins, which we propose facilitate neural induction by providing major anterior neural loci with a permissive regulatory topology.

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PRC2 facilitates the regulatory topology required for poised enhancer function during pluripotent stem cell differentiation [RNA-seq EED]

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PRC2 facilitates the regulatory topology required for poised enhancer function during pluripotent stem cell differentiation [ChIP-seq]

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PRC2 facilitates the regulatory topology required for poised enhancer function during pluripotent stem cell differentiation [4C-seq]

2017-02-11 | GSE89208 | GEO

Convergent evolution of complex regulatory landscapes and pleiotropy at Hox loci (4C-seq)

Project description:Hox genes are required during the morphogenesis of both vertebrate digits and external genitals.We investigated whether transcription in such distinct contexts involves a shared enhancer-containing landscape. We show that the same regulatory topology is used, yet with some tissue-specific enhancer-promoter interactions, suggesting the hijacking of a regulatory backbone from one context to the other. In addition, comparable organizations are observed at both HoxA and HoxD clusters, which separated through genome duplication in an ancestral invertebrate animal.We propose that this convergent regulatory evolution was triggered by the pre-existence of some chromatin architecture, thus facilitating the subsequent recruitment of the appropriate transcription factors. Such regulatory topologies may have both favored and constrained the evolution of pleiotropic developmental loci in vertebrates. Circular Chromosome Conformation Capture (4C-seq) samples from mouse digit cells and mouse genital tubercle cells performed as previously described (Noordermeer et al. 2011)

2014-11-21 | E-GEOD-62265 | biostudies-arrayexpress

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Project description:A comprehensive landscape of epigenomic events regulated by the Reelin signaling through activation of specific cohort of cis-regulatory enhancer elements (LRN-enhancers), which involves the proteolytical processing of the LRP8 receptor by the gamma-secretase activity and is required for learning and memory behavior All 4C-Seq experiments were designed to evaluate the physycal interaction between selected Fos promoter and putative regulatory enhancers regions

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