Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Homo sapiens

ABSTRACT: Single Cell RNA-Seq Analysis Reveals Fate Decision of Human Pre-cDC Determined by Transcription Factor Competition

PROVIDER: PRJNA351791 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR4726240.fastq.gz	Fastqsanger.gz
	SRR4726250.fastq.gz	Fastqsanger.gz
	SRR4726260.fastq.gz	Fastqsanger.gz
	SRR4726270.fastq.gz	Fastqsanger.gz
	SRR4726280.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 200

Similar Datasets

Single Cell RNA-Seq Analysis Reveals Fate Decision of Human Pre-cDC Determined by Transcription Factor Competition

Project description:Classic dendritic cells (cDCs) play a central role in the immune system and consist of two major subsets: CD141+ cDC (cDC1) and CD1c+ cDC (cDC2). The pre-cDCs is the immediate precursors to both cDC subsets. Previous studies showed that there were two pre-committed pre-cDC subpopulations. However, the key molecular drivers of pre-commitment in human pre-cDCs were not investigated. To address this question, we performed both single cell and bulk RNA sequencing (RNA-seq) of two cDC subsets and pre-cDCs. We inferred a list of sixteen candidate master regulator transcriptional factors (TFs) that can indeed separate pre-cDCs into two sub-populations, with one close to cDC1 and the other close to cDC2. More importantly, these two pre-cDC sub-populations are correlated with ratio of IRF8 to IRF4 expression level more than their individual expression level. Our results suggest the concept that the ratio of antagonistic TFs and their competition determine cDC subset differentiation fate.

2019-07-16 | GSE89322 | GEO

Cell fate decision by a morphogen-transcription factor-chromatin modifier axis

Project description:Cell fate decision by a morphogen-transcription factor-chromatin modifier axis

| PRJNA1015164 | ENA

CROP-seq of 2 month old brain organoids

Project description:To investigate the influence of transcription factor knockouts in cell fate decision-making, we performed a CROP-seq screen of 20 transcription factors in brain organoids.

2022-08-18 | E-MTAB-11999 | biostudies-arrayexpress

Cell competition regulates the fate decision of neural stem cells

Project description:Cell competition regulates the fate decision of neural stem cells

| PRJNA925106 | ENA

Chromatin Accessibility Dynamics During Zebrafish Early Embryogenesis At The Single-Cell Resolution

Project description:We performed plate-based single-nucleus experiments across multiple stages immediately after zygotic genome activation, including 1k, oblong, sphere and dome stages. The first point where cells become distinct at the chromatin level was determined. Many potential enhancers regulating the first cell fate decision were identified.

2023-10-13 | E-MTAB-13115 | biostudies-arrayexpress

High-throughput single cell gene expression analysis in primary blood stem and progenitor cells identifies new links between key transcriptional regulators

Project description:Cellular decision-making is mediated by a complex interplay of external stimuli with the intracellular environment, in particular transcription factor regulatory networks. Here we have determined the expression of a network of 18 key haematopoietic transcription factors (TFs) in 597 single primary blood stem and progenitor cells isolated from mouse bone marrow. We demonstrate that different stem/progenitor populations are characterised by distinctive TF expression states, and through comprehensive bioinformatic analysis reveal positively and negatively correlated TF pairings, including previously unrecognised relationships between Gata2, Gfi1 and Gfi1b. Validation using transcriptional and transgenic assays confirmed direct regulatory interactions consistent with a novel regulatory triad in immature blood stem cells, where Gata2 may function to modulate cross-inhibition between Gfi1 and Gfi1b. Single cell expression profiling therefore identifies network states and allows reconstruction of network hierarchies involved in controlling stem cell fate choices, and provides a blueprint for studying both normal development and human disease. Examination of Gata2 and Gfi1 binding patterns in murine mast cells

2013-04-24 | E-GEOD-42518 | biostudies-arrayexpress

Transcription factor cooperation and competition

Project description:Transcription factor cooperation and competition

| PRJNA933216 | ENA

Transcription factor KLF2 regulates cell fate decision of stem cells in infantile hemangioma

Project description:Transcription factor KLF2 regulates cell fate decision of stem cells in infantile hemangioma

| PRJNA998877 | ENA

Gene expression profile of mature plasmacytoid dendritic cells (PDC) after the deletion of transcription factor E2-2

Project description:The interferon-producing plasmacytoid dendritic cells (PDC) share common progenitors with antigen-presenting classical dendritic cells (cDC), yet they possess distinct morphology and molecular features resembling those of lymphocytes. It is unclear whether the unique cell fate of PDC is actively maintained in the steady state. We report that the deletion of transcription factor E2-2 from mature peripheral PDC caused their spontaneous differentiation into cells with cDC properties. This included the loss of PDC markers, increase in MHC class II expression and T cell priming capacity, acquisition of dendritic morphology and induction of cDC signature genes. Genome-wide chromatin immunoprecipitation revealed direct binding of E2-2 to key PDC-specific and lymphoid genes, as well as to certain genes enriched in cDC. Thus, E2-2 actively maintains the cell fate of mature PDC and opposes the “default” cDC fate, in part through direct regulation of lineage-specific gene expression programs. Inducible deletion of E2-2 (Tcf4) has been performed by administering tamoxifen to conditional E2-2flox/flox Rosa26-CreER+ mice or to E2-2flox/flox Rosa26-CreER- control littermates. Four or six days later total splenocytes were isolated, pooled from 2-3 mice and PDC (CD11b- B220+ CD11clow Bst2+) were isolated by sorting. Global gene expression profiles of E2-2-deficient (null) and control (Ctrl) PDC were compared using Affymetrix microarrays (GPL1261).

2010-12-21 | E-GEOD-24726 | biostudies-arrayexpress

Binding targets of transcription factor E2-2 in human plasmacytoid dendritic cells

2010-12-21 | GSE24740 | GEO