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Homo sapiens strain:fibroblast | isolate:UV #1,UV #2 | breed:1 | cultivar:DMEM

ABSTRACT: Homo sapiens strain:fibroblast | isolate:UV #1,UV #2 | breed:1 | cultivar:DMEM Raw sequence reads

PROVIDER: PRJNA352112 | ENA |

REPOSITORIES: ENA

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Homo sapiens strain:fibroblast | isolate:UV #1,UV #2 | breed:1 | cultivar:DMEM

Project description:Homo sapiens strain:fibroblast | isolate:UV #1,UV #2 | breed:1 | cultivar:DMEM Raw sequence reads

| PRJNA352111 | ENA

Homo sapiens strain:fibroblast | isolate:UV #1,UV #2 | breed:1 | cultivar:DMEM

Project description:Homo sapiens strain:fibroblast | isolate:UV #1,UV #2 | breed:1 | cultivar:DMEM Raw sequence reads

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MIRA-Seq analysis of bovine dermal fibroblasts isolated from two breeds of cattle, Holstein (dairy breed) and Angus (beef breed).

Project description:We report the results of MIRA-Seq based high-throughput profiling of the bovine dermal fibroblast methylome from two different breeds of cattle (n=4/breed) to determine the breed-dependent differences in methylation.

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Capra hircus isolate:goat lip fibroblast | breed:animals | cultivar:cell

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Project description:In the present study we have used a new custom made Affymetrix GrapeGen GeneChip to investigate gene expression responses of grapevine cultivar Malbec to one dose of biologically effective UV-B radiation (4.75 kJ m-2 d-1), administered at two different intensities (16 h, to 8.25 µW cm-2 or 4 h, to 33 µW cm2 UV-B).

2010-12-21 | E-MEXP-2541 | biostudies-arrayexpress

RNAseq analysis of Lactuca sativa leaf under UV treatment

Project description:Baby leaves of lettuce (Lactuca sativa L.) are widely used to produce ready-to-eat salads all around the year. For this purpose, lettuce plants are cultivated under plastic tunnels and harvested at a very early growth stage, between 30-45 days after sowing depending on the season, cultivar and site of cultivation. Usually, the plastic film covering the cultivation tunnels transmits only a very small amount of the solar UVB radiation and partially attenuates UVA radiation. We used UV treatment post-harvest of fresh-cut lettuce baby leaves to compare the efficiency of two UV emission wavebands centred at 306 and 366 nm (achieved by employing narrow-band UV LEDs sources) to identify putative transcriptomic signatures. The analyses have been carried out supported by and in the frame of Project @CNR 2021, Title "EnhAncement of Natural anTIviral Compounds in ready- to-eat Vegetables", Acronym: ANTI-CoV to Dr. G. Agati. IDP-NAHEP, ICAR, Govt of India to A.R.B.

2025-07-04 | GSE292446 | GEO

Homo sapiens breed:eukaryotic breed

Project description:Homo sapiens breed:eukaryotic breed Random survey

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Tissue-specific gene expression patterns in fibroblast cell lines

Project description:Fibroblast cell lines from the dermis of the skin, the oral cavity, and the lungs were cultured in serum free DMEM and gene expression patterns were examined by RNA sequencing to determine tissue specific gene expression and programs expressed in fibroblasts in different tissues.

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DMEM treated WT and PKBa -/- MEFs

Project description:Mouse embryonic fibroblasts (MEFs) were generated from 13.5-day-old embryos obtained from heterozygous PKBa mice intercrosses (Yang et al., 2003). Briefly, after dissection of head and visceral organs for genotyping, embryos were minced and trypsinized for 30 min at 37°C. Embryonic fibroblasts were then plated and maintained in Dulbecco’s Modified Eagle Medium (DMEM) with 10% foetal calf serum (FCS) (Life Technologies), 100 units/ml of penicillin and 100 mg/ml of streptomycin at 37°C in an atmosphere of 5% CO2. All experiments were performed with wild-type and PKBa-/- MEFs between 15-20 passages. To induce adipocyte differentiation, 2-day-postconfluent cells (day 0) were treated with DMEM supplemented with 10% FCS, 8 mg/ml biotin, 4 mg/ml pantothenate, 0.5 mM 3-isobutyl-1-methylxanthine, 1 mM dexamethasone and 10 mg/ml insulin (all from Sigma). Total RNA was extracted from cells using TRIzol (Invitrogen) according to the manufacturer’s instructions. Keywords: repeat

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Transcriptome analysis between wild-type and DM1-affected fibroblast.

Project description:Human skin fibroblast cell lines from 3 normal individuals and 3 individuals with DM1 in one pedigree were obtained from the biobank of Shanghai East Hospital. Human skin fibroblast cells were grown in DMEM (Life Technologies), NaPyr, GlutaMAX, β-mercaptoethanol and 10% foetal bovine serum (FBS) supplemented medium as previously described (Richner et al. 2015). Cells were collected after 80% confluency with TRIzol reagent and stored at -80℃ for RNA-seq and RT-PCR.

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