Project description:Deep characterization of a large series of splenic diffuse red pulp lymphomas DNA from 5 tumor samples, corresponding to 4 cases, were analyzed with Affymetrix SNP 6.0 platform for copy number alteration study.
Project description:To investigate the biological differences between HIV-, HIV+/ART-experienced and HIV+/ART-naive diffuse large B-cell lymphoma, we performed whole exome sequencing of 30 pre-treatment formalin-fixed paraffin-embedded (FFPE) whole lymph node biopsies of diffuse large B-cell lymphoma.
Project description:Splenic mononuclear phagocytes are critical in various homeostatic processes as well as immune responses. We employed single-cell RNA sequencing to investigate mononuclear phagocytic cell subsets, including splenic red pulp macrophages, monocytes, and dendritic cells in the spleen, with a focus on how Notch2 and its nuclear mediator Rbpj regulate these subsets. Our study offers a comparative analysis of splenic mononuclear phagocytes and specifically red pulp macrophages examining phagocytes isolated from myeloid Notch2 (N2ΔCx3cr1)-, and Rbpj (RbpjΔCx3cr1)-deficient mice or control animals. Our findings may provide further insights into the heterogeneity and development of the splenic mononuclear phagocytic system including splenic macrophages and monocytes.
Project description:To compare the splenic macrophages between SIRPα-knockout mice and WT mice, we performed a complete transcript profiling of the splenic red pulp macrophages from SIRPα-KO mice compared to WT mice using mRNA microarray as a discovery platform. SIRPα-KO mice and WT mice were kept under the same condition. Macrophages were isolated from spleen red pulp of SIRPα-KO mice and WT mice. RNA was then isolated from the same number of freshly isolated macrophages.
