Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mus musculus

ABSTRACT: RNA-seq of FACS sorted neurons overexpressing miR-203 in wild-type mice

PROVIDER: PRJNA354340 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR5031380_1.fastq.gz	Fastqsanger.gz
	SRR5031380_2.fastq.gz	Fastqsanger.gz
	SRR5031390_1.fastq.gz	Fastqsanger.gz
	SRR5031390_2.fastq.gz	Fastqsanger.gz
	SRR5031381_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 44

Similar Datasets

RNA-seq of FACS sorted neurons overexpressing miR-203 in wild-type mice

Project description:Purpose: The goal of this study was to assess gene expression changes in neurons overexpressing miR-203 in mouse frontal cortex.

2018-08-28 | GSE89981 | GEO

Gene expression analysis in islets from miR-203 TG mice [scRNA-seq]

Project description:To investigate the effect of miR-203 in type 2 diabetes, target genes of miR-203 need to be investigated. The β cell specific miR-203 transgene (miR-203 TG) mice was constructed, and scRNA-seq was then performed on mouse islets.

2023-05-19 | GSE232474 | GEO

Gene expression analysis in islets from miR-203 TG mice [RNA-seq]

2023-05-19 | GSE232473 | GEO

Expression data of mRNA from wild-type A549 cells and microRNA-203 knockout A549 cells during H5N1 infections

Project description:MicroRNA-203 was up-regulated markedly upon H5N1 virus infection. To identify the potential target genes of miR-203, we constructed a miR-203 knockout A549 cell line. Then wild-type and miR-203 knockout A549 cells were mock-infected or infected with H5N1 virus for 48h. The Agilent Whole Human Genome Oligo Microarray was performed to analyze the mRNA expression profiling. Meanwhile, the online tool TargetScanHuman (http://www.targetscan.org/vert_71/) was used to predict biological targets of miR-203. We combined the predicted genes with the genes differentially expressed in wild-type and miR-203 knockout A549 cells, and preliminarily identified some candidate mRNAs. Then more experiments were performed to further verify these target genes, such as dual-luciferase reporter assay, quantitative real-time PCR or Western blot analysis.

2018-05-09 | GSE107089 | GEO

Genes regulated by miR-203 in breast cancer cell line MDA-MB-231

Project description:To investigate the mechanism through which miR-203 inhibited the breast cancer cell invasion, we overpression miR-203 in MDA-MB-231 cell line and performed a microarray to examine the genes which maybe targeted and down-regulated by miR-203. We made a stable cell line(MB231/miR-203) which overexpressing miR-203 and a control cell line (MB231/EV) which expressing a contrlol vector. Total RNA were extracted from these two cell lines and subject to microarray.

2013-05-01 | E-GEOD-44239 | biostudies-arrayexpress

Global proteome of Atg5flox:Slc32a1-Cre:tdTomato FACS sorted WT and KO neurons

Project description:The experiment was performed to identify autophagy targets in wildtype and autophagy-deficient forebrain inhibitory neurons. Therefore, neurons were isolated from the cortex, hippocampus and striatum of 2-3 weeks old Atg5flox/flox:Slc32a1-Cretg/wt:tdTomato+ (KO) and Atg5wt/wt:Slc332a1-Cretg/wt:tdTomato+ (WT) mice. Neurons in suspension were FACS sorted and inhibitory forebrain neurons expressing tdTomato were forwarded to global proteome analysis assessed by LC-MS/MS.

2022-11-15 | PXD031535 | Pride

Global proteome of Atg5flox:CamKIIα-Cre:tdTomato FACS sorted WT and KO neurons

Project description:The experiment was performed to identify autophagy targets in wildtype and autophagy-deficient forebrain excitatory neurons. Therefore, neurons were isolated from the cortex, hippocampus and striatum of 2-3 weeks old Atg5flox/flox:CamKIIα-Cretg/wt:tdTomato+ (KO) and Atg5wt/wt:CamKIIα-Cretg/wt:tdTomato+ (WT) mice. Neurons in suspension were FACS sorted and excitatory forebrain neurons expressing tdTomato were forwarded to global proteome analysis assessed by LC-MS/MS.

2022-11-15 | PXD031552 | Pride

RNA sequencing of Mus musculus T helper cell type 2 with retroviral overexpression of different genes

Project description:T cells were extracted from spleens of CBLA mice and activated in vitro with CD3/28 on day 0. They were spinoculated with retroviruses overexpressing a gene of interest on day 1. On day 4, BFP-positive (infected) cells were FACS sorted and measured by a variant of SMART-seq2

2018-11-27 | E-MTAB-7260 | biostudies-arrayexpress

Quantitative Analysis of Wild Type and miR-203 KO transcriptomes in the rat endometrial adenocarcinoma cell line RUCA-I

Project description:Purpose: identification of mRNAs that are potential targets of miR-203 in the endometrium and endometrial carcinoma Methods: mRNA profiles of three batches of wild-type (WT) and three independently generated miR-203 knockout (miR-203 KO) RUCA-I cells were produced by deep sequencing, using Illumina HiSeq 2500. The sequence reads that passed quality filters were analyzed at the transcript isoform level with TopHat followed by Cufflinks. Results: Using an optimized data analysis workflow, we mapped between 30 and 50 million sequence reads per sample to the rat genome (build rn6) and identified 26751 transcripts of which 1591 are differentially expressed in WT and miR-203 KO cells (p<0.05).

2017-03-10 | GSE95850 | GEO

ChIP-seq of Mus musculus T helper cell type 2 with retroviral overexpression of Bhlhe40 and Pparg

Project description:T cells were extracted from spleens of CBLA mice and activated in vitro with CD3/28 on day 0. They were spinoculated with retroviruses overexpressing a 3xFLAG-gene of interest on day 1. On day 4, BFP-positive (infected) cells were FACS sorted and measured by ChIPmentation. For input ChIP controls, see E-MTAB-6276

2018-11-23 | E-MTAB-7258 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data