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Deinococcus geothermalis

ABSTRACT: Deinococcus geothermalis overview

PROVIDER: PRJNA35515 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR11935554_1.fastq.gz	Fastqsanger.gz
	SRR11935554_2.fastq.gz	Fastqsanger.gz
	SRR11935555_1.fastq.gz	Fastqsanger.gz
	SRR11935555_2.fastq.gz	Fastqsanger.gz

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RNA-Seq analysis among dps-disrupted mutants in Deinococcus geothermalis

Project description:Transcriptomic analysis was performed in Deinococcus geothermalis wild-type and three mutants including dgeo_0257, dgeo_0281, and double knockout (DK) mutants. dgeo_0281 is a major Dps-coding gene and dgeo_0257 is a novel Dps candidate. Thus, to detecte physiological functions of both Dps-encoded genes, each gene-disrupted mutant and DK mutant was constructed and performed RNA-Seq analysis. This result support better understanding for Dps roles in radiation resistant bacterium through up-regulated and down-regulated genes profile.

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Transcriptomic analysis of a putative LysR family regulator, a putative Dps, and a cystine importer disrupted mutants in radiation resistant bacterium Deinococcus geothermalis

Project description:Here, we compared gene expression level by RNA-Seq analysis among a wild-type (WT) Deinococcus geothermalis strain, a dgeo_2840 of LysR family member gene (Δdgeo_2840), a putative Dps dgeo_0257 (Δdgeo_0257), and a cystine importer dgeo_1986-1987 gene disrupted strain (Δdgeo_1986-1987). From this transcriptomic data, we detected dgeo_2840, dgeo_0257, and dgeo_1986-1987 controlling genes which were somehow up-regulated and/or down-regulated. In particular, Deinococcus geothermalis has a total of 73 insertion sequences (ISs) in genomes, and some of them are actively transposed to other loci with replicative mode by the oxidative stress of hydrogen peroxide treatment.

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