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Sordaria macrospora

ABSTRACT: Gene expression in developmental mutants of Sordaria macrospora

PROVIDER: PRJNA357259 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5101400.fastq.gz	Fastqsanger.gz
	SRR5101401.fastq.gz	Fastqsanger.gz
	SRR5101402.fastq.gz	Fastqsanger.gz
	SRR5101393.fastq.gz	Fastqsanger.gz
	SRR5101403.fastq.gz	Fastqsanger.gz

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Gene expression in developmental mutants of Sordaria macrospora

Project description:The aim of this analysis was to analyze gene expression in developmental mutants of the filamentous ascomycete Sordaria macrospora. RNA was isolated from total mycelia, or young fruiting bodies (protoperithecia) were isolated by laser microdissection and RNA was extracted, amplified and used for RNA-sequencing.

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Project description:The Sordaria macrospora nox1 mutant has a block in sexual development at the stage of protoperithecia formation, and therefore is sterile. The aim of this study was to determine the transcriptome of microdissected protoperithecia from the mutant, as well as the transcriptomes of total RNA from the mutant and the wild type for comparison. The data were then compared with transcriptome data from protoperithecia of the wild type and the developemental mutant pro1; the corresponding data were generated in a previous study (Teichert et al. 2012, BMC Genomics 13: 511, GEO accession GSE33668).

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