Daphnia pulex
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PROVIDER: PRJNA357527 | ENA |
REPOSITORIES: ENA
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Project description:Phenotypic plasticity of temperature induced Daphnia gene expression experiment 1
| PRJNA356699 | ENA
Project description:Phenotypic plasticity of temperature induced Daphnia gene expression
| PRJNA357371 | ENA
Project description:Daphnia pulex RNA-Seq overall temperature response
| PRJNA1251057 | ENA
Project description:This SuperSeries is composed of the following subset Series: GSE25841: Evolutionary Diversification of Duplicated Genes; Experiment A GSE25843: Evolutionary Diversification of Duplicated Genes; Experiments B-I, M-P GSE25845: Evolutionary Diversification of Duplicated Genes; Experiments B-I GSE25850: Evolutionary Diversification of Duplicated Genes; Experiment J GSE25851: Evolutionary Diversification of Duplicated Genes; Experiment L, K GSE25852: Empirical Annotation of the Daphnia pulex genome; Experiment B GSE25855: Empirical Annotation of the Daphnia pulex genome; Experiment A GSE25856: Empirical Annotation of the Daphnia pulex genome; Experiment C Refer to individual Series
2011-02-01 | E-GEOD-25823 | biostudies-arrayexpress
Project description:Changing global climates present threats to aquatic ecosystems with events such as heatwaves and prolonged increased temperatures altering habitats and affecting species survival. Aquatic organisms are particularly vulnerable to these elevated temperatures often due to a lack of suitable migration routes. A decrease in population abundance and survival in species occupying lower trophic levels may have cascading effects on the health of an ecosystem. In this study, we investigated the fitness (fecundity) and transcriptional response of Daphnia pulex, a common grazer in lentic freshwater systems, to sublethal temperature stress. D. pulex were exposed to control (20℃) and elevated sublethal (25℃) temperature to simulate an increased water temperature from a mild heat wave for 168 hours. Our findings indicate a dynamic transcriptional response to elevated temperatures. Notably, differential gene expression between the control and temperature-elevated treatment increased throughout the experiment with a three-fold increase in counts of DEGs at 168 hours as compared to 96 hours. D. pulex within the experimental treatment had higher mean cumulative offspring produced compared to the control treatment. Given D. pulex’s role as a foundational species in aquatic food webs, the observed transcriptional response provides insight into the potential for both plastic and adaptive responses in the face of environmental change.
2025-12-18 | GSE296043 | GEO
Project description:Background Daphnia species reproduce by cyclic parthenogenesis involving both sexual and asexual reproduction. The sex of the offspring is environmentally determined and mediated via endocrine signalling by the mother. Interestingly, male and female Daphnia can be genetically identical, yet display large differences in behaviour, morphology, lifespan and metabolic activity. Our goal was to integrate multiple omics datasets, including gene expression, splicing, histone modification and DNA methylation data generated from genetically identical female and male Daphnia pulex under controlled laboratory settings with the aim of achieving a better understanding of the underlying epigenetic factors that may contribute to the phenotypic differences observed between the two genders. Results In this study we demonstrate that gene expression level is positively correlated with increased DNA methylation, and histone H3 trimethylation at lysine 4 (H3K4me3) at predicted promoter regions. Conversely, elevated histone H3 trimethylation at lysine 27 (H3K27me3), distributed across the entire transcript length, is negatively correlated with gene expression level. Interestingly, male Daphnia are dominated with epigenetic modifications that globally promote elevated gene expression, while female Daphnia are dominated with epigenetic modifications that reduce gene expression globally. For examples, CpG methylation (positively correlated with gene expression level) is significantly higher in almost all differentially methylated sites in male compared to female Daphnia. Furthermore, H3K4me3 modifications are higher in male compared to female Daphnia in more than 3/4 of the differentially regulated promoters. On the other hand, H3K27me3 is higher in female compared to male Daphnia in more than 5/6 of differentially modified sites. However, both sexes demonstrate roughly equal number of genes that are up-regulated in one gender compared to the other sex. Since, gene expression analyses typically assume that most genes are expressed at equal level among samples and different conditions, and thus cannot detect global changes affecting most genes. Conclusions The epigenetic differences between male and female in Daphnia pulex are vast and dominated by changes that promote elevated gene expression in male Daphnia. Furthermore, the differences observed in both gene expression changes and epigenetic modifications between the genders relate to pathways that are physiologically relevant to the observed phenotypic differences.
2019-10-18 | GSE124427 | GEO
Project description:Empirical Annotation of the Daphnia pulex genome Experiment B
| PRJNA142495 | ENA