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Companilactobacillus alimentarius DSM 20249

ABSTRACT: Whole Genome sequencing and assembly of Lactobacillus alimentarius DSM 20249T

PROVIDER: PRJNA357929 | ENA |

REPOSITORIES: ENA

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Project description:Investigation of whole genome gene expression level changes in Lactococcus lactis KCTC 3769T,L. raffinolactis DSM 20443T, L. plantarum DSM 20686T, L. fujiensis JSM 16395T, L. garvieae KCTC 3772T, L. piscium DSM 6634T and L. chungangensis CAU 28T . This proves that transcriptional profiling can facilitate in elucidating the genetic distance between closely related strains. A one chip study using total RNA recovered from of L. raffinolactis DSM 20443T, L. plantarum DSM 20686T, L. fujiensis JSM 16395T, L. garvieae KCTC 3772T, L. piscium DSM 6634T and L. chungangensis CAU 28T . For the the transcriptome of of L. raffinolactis DSM 20443T, L. plantarum DSM 20686T, L. fujiensis JSM 16395T, L. garvieae KCTC 3772T, L. piscium DSM 6634T and L. chungangensis CAU 28T was analyzed using the Lactococcus lactis KCTC 3769T microarray platform

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Companilactobacillus alimentarius Y4 alleviates salmonella-induced intestinal inflammation by regulating gut microbiota and its metabolites

Project description:The emergence of ciprofloxacin-resistant Salmonella poses a significant challenge to the development of new drugs. Probiotics that inhibit ciprofloxacin-resistant Salmonella offer a safer alternative with fewer side effects than chemical antibiotics and drugs. Here, a strain of Companilactobacillus alimentarius Y4, isolated from kimchi, demonstrated strong antibacterial activity against Salmonella C1, E. coli and other intestinal pathogens. The results of the biological characteristics experiment show that the survival rate of C. alimentarius Y4 was 80% at pH 3.0 and 87.13% at 0.3% bile salt concentration. It retained antibacterial activity even after exposure to pepsin, trypsin, protease K, and catalase. Additionally, C. alimentarius Y4 down-regulates the inflammatory cytokines IL-1β, TNF-α, and IL-6 while upregulating the anti-inflammatory cytokine IL-10. It effectively repaired intestinal damage caused by ciprofloxacin-resistant Salmonella C1. Gut flora analysis revealed that C. alimentarius Y4 colonized the intestine and increased the abundance of beneficial gut microbes such Lachnospiraceae bacterium-COE1 and Muribaculaceae-CAG-485. Furthermore,analysis of intestinal metabolites indicated that C. alimentarius Y4 Up-regulate L-leucine, L-lysine and Tryptophan and other metabolites and then restored intestinal metabolic balance. In summary, C. alimentarius Y4 by regulating inflammatory cytokines, repairing intestinal damage, rebalancing gut flora, and correcting intestinal metabolic disorders,it is holds promise as an effective probiotic for managing intestinal inflammation caused by ciprofloxacin-resistant Salmonella. This study lays a foundation for the clinical development of probiotic products.

2025-07-26 | MTBLS12777 | MetaboLights

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