Oryza sativa
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA358014 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR5120080.fastq.gz | Fastqsanger.gz | |||
| SRR5120081.fastq.gz | Fastqsanger.gz | |||
| SRR5120082.fastq.gz | Fastqsanger.gz | |||
| SRR5120083.fastq.gz | Fastqsanger.gz | |||
| SRR5120084.fastq.gz | Fastqsanger.gz |
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Similar Datasets
Project description:Brown planthopper (BPH; Nilaparvata lugens) is a phloem feeding insect which is one of the most serious threats to rice crops in many countries throughout Asia. 1H NMR spectroscopy, combined with chemometrics, was used to analyze the polar metabolome from leaf extracts of Thai Jasmine rice (brown planthopper (BPH)-susceptible KD) and its BPH resistant isogenic lines (BPH-resistant IL7 and BPH-resistant+ IL308 varieties) with and without BPH infestation at various time points (days 1, 2, 3, 4 and 8). Physiological changes of the rice isogenic lines were different based on the quantitative trait loci of BPH resistance. Multivariate models were capable of distinguishing between the susceptible and the resistant rice varieties throughout the infestation. The concentration of 10 metabolites were significantly altered (p < 0.05) between the infested and the control groups of each examined rice variety. Metabolic pathway analysis suggested that BPH infestation could perturb transamination during the early stages of infestation (days 1–3) for all rice varieties. In addition, the IL7 and IL308 varieties responded earlier (day 3) than the KD variety (day 8) by perturbing amino acid metabolism, shikimate and gluconeogenesis pathways. By day 8 of the infestation, the KD cultivar responded by activating the amino acid-mediated-de novo pathway whereas the IL308 variety activated the purine and pyrimidine compound-mediated-salvage pathway for nucleotide biosynthesis. This study has identified, for the first time, several potential metabolic pathways for acclimatization and defense mechanisms against BPH infestation. These findings provide a valuable, first insight into BPH resistance mechanisms in Thai Jasmine rice.
2016-11-25 | MTBLS151 | MetaboLights
Project description:The aim of this study was to analyze potential brown planthopper (BPH) resistant genes in Rathu Heenati (RHT) by Affymetrix whole rice genome array,BPH susceptible and resistant rice varieties of TN1（Taichung Native 1）as control. All the resistant related genes derived from RHT will be analyzed according to the SSR markers interval flanked on the chromosome 3, 4, 6 and 10. It will be benefit to the gene clone and marker assistant breeding for Bph3 gene in the near future. We used microarrays to detail the global differential gene expression before and after brown planthopper attack in two different varieties, and identified distinct classes of high enriched genes induced by BPH or constituent in RHT
2011-12-31 | GSE29967 | GEO
Project description:The aim of this study was to analyze potential brown planthopper (BPH) resistant genes in Rathu Heenati (RHT) by Affymetrix whole rice genome array,BPH susceptible and resistant rice varieties of TN1（Taichung Native 1）as control. All the resistant related genes derived from RHT will be analyzed according to the SSR markers interval flanked on the chromosome 3, 4, 6 and 10. It will be benefit to the gene clone and marker assistant breeding for Bph3 gene in the near future. We used microarrays to detail the global differential gene expression before and after brown planthopper attack in two different varieties, and identified distinct classes of high enriched genes induced by BPH or constituent in RHT The 2nd to 3rd instar nymphs of BPH were transferred to tillering stage seedings (10 BPH nymphs per plant) in a box covered with nylon-mesh. Stems of the rice plant infected by BPH were collected at 0h (T0), 8h (T8), 24h (T24) after BPH attack, total RNA were extracted for the microarray hybirdlization.
2011-12-31 | E-GEOD-29967 | biostudies-arrayexpress
Project description:Infestation with white-backed planthopper (WBPH) to rice caused induced resistance to rice pathogens but brown planthopper (BPH) infestation induce weaker resistance to rice pathogens. We compared changes in gene expression in rice plants infested with WBPH and BPH to gain some insight into the WBPH-induced resistance to rice pathogens. An analysis, using microarrays, of gene expression in rice plants infested with these planthoppers revealed that WBPH infestation caused high induction of many defense-related genes including pathogenesis-related (PR) genes than BPH infestation. Furthermore, hydroperoxide lyase 2 (OsHPL2) which is an enzyme to produce C6 volatiles was induced by WBPH infestation, but not by BPH infestation. Keywords: response to herbivory; induced resistance
2007-08-21 | GSE8811 | GEO
Project description:Genome sequence of brown planthopper resistant rice cultivar
| PRJDB9518 | ENA
Project description:Purpose: The goal of our study is to compare two different ecotypes of Oryza sativa L., PHS-susceptible rice trait and PHS-resistant rice trait under three different maturation stages in rice seed embryo with profile of miRNA-seq. Methods: Oryza sativa. L miRNA profiles of two different ecotypes with 3 different maturation stages of rice seed embryo were generated by NGS, in duplicate, following Illumina NGS workflow. Results: We found the differentially expressed microRNAs between PHS-susceptible rice trait and PHS-resistant rice trait according to the three different seed maturation stages. Target transcripts of differentially expressed microRNAs have been predicted via psRNATarget web server, and a part of those target genes are likely to be regulated by microRNAs, affecting overall responses to heat stress and the regulation of seed dormancy during maturation. Conclusions: Our study represents the analysis of rice seed small RNAs, specifically microRNAs, under two different ecotypes, three different seed maturation stages in rice seed embryo. Our results show that microRNAs are involved in response to heat stress and the regulation of seed dormancy. This study will provide a foundation for understanding dynamics of seed dormancy during the seed development and overcoming pre-harvest sprouting.
2021-07-04 | GSE175561 | GEO
Project description:Here, we reported the quantitative analysis of brown planthopper (BPH) interactions with rice stem tissue by iTRAQ proteomic techonology.The results obtained from this work not only aimed to provide a new clues that will facilitate better understanding of complex molecular and cellular events in BPH infestation, but also explored the regulatory roles of HSP20 for breeding BPH resistant rice.
2019-11-14 | PXD008926 | Pride
Project description:To explore the molecular mechanisms underlying the rice plant-mediated interaction between brown planthopper (BPH) and striped stem borer (SSB), gene expression changes in rice plant response to infestation by SSB, BPH or both and control were analyzed by RNA-seq.
2021-02-28 | GSE167872 | GEO
Project description:Rice infection with RRSV and RGSV causes severe symptoms and is transmitted by the brown planthopper, which feeds on the base of the rice stem and spreads the virus. We analyzed cell heterogeneity at the base of the rice stem after virus infection by single cell RNA sequencing (scRNA-seq).
2025-10-31 | GSE222584 | GEO
Project description:Although Cochliobolus miyabeanus is an important fungal leaf pathogen on rice plants worldwide, it is largely neglected by molecular plant phytopathologists. To shed new light on the molecular and genetic basis of the rice – C. miyabeanus interaction, we compared the transcriptome of rice leaves 12h post inoculation to uninfected leaves. Even though usable sources of resistance against brown spot disease caused by C. miyabeanus are scarce, silicon application emerges as a sustainable protection strategy. Many articles report the beneficial effect of silicon on brown spot resistance. however the underlying mechanisms remain largely unclear. The influence of silicon application on the transcriptome of healthy and infected rice leaves 12hpi was compared as well in an attempt to disentangle the modulation of silicon-induced brown spot resistance.
2014-02-25 | GSE55330 | GEO