Project description:A whole genome microarray approach has been engaged in the heterothallic euascomycete Podospora anserina to identify genes that are differentially expressed beetwen a wild type kinetics of sexual reproduction and two mutant strains ∆RID and ∆SMR1.
Project description:A whole genome microarray approach has been engaged in the heterothallic euascomycete Podospora anserina to identify genes that regulate the key steps of its sexual development. A time-course transcriptional study of 10 developmental stages from fertilization to ascospore maturation has been performed. More than half of the genome (58% of the 10540 CDS) displayed a significant change of transcript levels between at least two developmental stages of the kinetic (i.e. on all comparisons between the 10 developmental stages).
Project description:Mutations in the gene for the mitochondrial matrix protease CLPP can cause human Perrault syndrome, which is characterized by male and female infertility, progressive sensorineural deafness, ataxia and leukoencephalopathy. This gene encodes a peptidase that is conserved since bacteria and localizes to mitochondrial matrix in eukaryotes. To compare and validate mouse and human findings, the filamentous fungus Podospora anserina was cultured and protein extracts were analyzed.
