Project description:We aim to understand the global transcriptional response of hybrid poplar NM6 (Populus nigra x Populus maximoviczii) to infection by two biotrophic Melampsora fungi, M. larici-populina and M. medusae f. sp. deltoidae separately and simultanously. Transcript profilling revealed 699 differentially expressed genes whose expression level was equal or higher than 2-fold between infected and noninfected tissues. In the mixed infection, our results showed an additive response of the two pathogens. Genes regulated as a result of Melampsora infection were generally induced and mainly involved in primary/secondary metabolism that include cell wall reinforcement and lignification, defense-related processes like pathogenesis-related and stress response, signal perception and transduction mechanisms and regulation of transcription. Keywords: Stress response
Project description:Dikaryotic rust fungi maintain two distinct haploid nuclei for most of their life cycle, making their large, repeat-rich genomes difficult to assemble and phase. Here we present haplotype-phased, near chromosome-scale genome assemblies for the poplar rust pathogens Melampsora larici-populina 98AG31 and Melampsora allii-populina 12AY07, generated using PacBio HiFi sequencing and Hi-C-guided scaffolding. For each species, we resolve 18 chromosomes per haplotype, providing the first chromosome-level representations of poplar rust fungal species. M. larici-populina diploid assembly spans ~203 Mb, while M. allii-populina reaches ~416 Mb, with high completeness and strong collinearity between haplotypes.
Project description:Investigation of whole genome gene expression level in motile strain of Sphingomonas. sp A1 All flagellar genes in motile strain of Sphingomonas. sp A1 are highly transcribed.