Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Freshwater metagenome

ABSTRACT: Metagenomic analysis of viruses in reclaimed water

PROVIDER: PRJNA36649 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR014584.fastq.gz	Fastqsanger.gz
	SRR014585.fastq.gz	Fastqsanger.gz
	SRR014586.fastq.gz	Fastqsanger.gz
	SRR014587.fastq.gz	Fastqsanger.gz
	SRR014588.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

freshwater metagenome

Project description:Freshwater Viruses Metagenomic assembly

| PRJNA288501 | ENA

freshwater metagenome

Project description:Metagenomic analysis of Vibrio cholerae contaminated fresh water

| PRJNA33243 | ENA

freshwater metagenome

Project description:Bacterioplankton in a recreational lake with reclaimed water Targeted Locus (Loci)

| PRJNA260704 | ENA

Cachexia - Non-Cachexia Metagenome Analysis

Project description:Cancer cachexia has been linked to gut bacterial alterations, but alterations of gut viruses, mostly bacteriophages, have not yet been explored. We performed shotgun metagenomic sequencing of DNA from stool samples of 78 cachectic and 42 non-cachectic cancer patients. K-mer-based matching to reference databases revealed abundance variations of bacteria and viruses. Beyond bacterial alterations, cachectic patients exhibited significantly lower bacteriophage abundance, predominantly affecting Caudovirales and Siphoviridae species (double-stranded DNA) but also Inoviridae and Microviridae families (single-stranded DNA).

| EGAS00001007156 | EGA

Project description:Metagenomic sequences of reclaimed water-dominant river

| PRJNA1018119 | ENA

Evaluation of Protein Extraction Methods to Improve Meta-Proteomics Analysis of Treated Wastewater Biofilms

Project description:Metaproteomics can be used to study functionally active biofilm-based bacterial populations in reclaimed water distribution systems, which in turn result in bacterial regrowth that impacts the water quality. However, existing protein extraction methods have differences in their protein recovery and have not been evaluated for their efficacies in reclaimed water biofilm samples. In this study, we first evaluated six different protein extraction methods with diverse chemical and physical properties on a mixture of bacterial cell culture. Based on a weighting scores-based evaluation, the extraction protocols in order of decreasing performance are listed as B-PER > RIPA > PreOmics > SDS > AllPrep > Urea. The highest four optimal methods on cell culture were further tested against treated wastewater non-chlorinated and chlorinated effluent biofilms. In terms of protein yield, our findings showed that RIPA performed the best; however, the highest number of proteins were extracted from SDS and PreOmics. Furthermore, SDS and PreOmics worked best to rupture gram-positive and gram-negative bacterial cell walls. Considering the five evaluation factors, PreOmics obtained highest weighted score, indicating its potential effectiveness in extracting proteins from biofilms. This study provides the first insight into evaluating protein extraction methods to facilitate metaproteomics for complex reclaimed water matrices.

2023-08-04 | PXD042292 | Pride

freshwater metagenome

Project description:Pearl River SS-surface water metagenomic and metatranscriptomic

| PRJNA320168 | ENA

freshwater metagenome

Project description:Cross-Omics analysis of Microcyctis aeruginosa and its viruses

| PRJDB7435 | ENA

freshwater metagenome

Project description:shogun metagenomic analysis in HKU

| PRJDB3780 | ENA

freshwater metagenome

Project description:Metagenomic analysis in Bangkok, Thailand