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Paracoccus denitrificans

ABSTRACT: Paracoccus denitrificans overview

PROVIDER: PRJNA36719 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	DRR017813.fastq.gz	Fastqsanger.gz
	DRR017814.fastq.gz	Fastqsanger.gz
	DRR327458_1.fastq.gz	Fastqsanger.gz
	DRR327458_2.fastq.gz	Fastqsanger.gz

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Transcriptome analysis of Paracoccus denitrificans during Cu-limitation

Project description:Transcriptional profiling of Paracoccus denitrificans PD1222 wild type grown to mid-exponential phase in minimal media with either 13 uM (Cu-H) or 0.5 uM (Cu-L) Cu regimes. The goal was to define the effects of Cu-limitation on denitrification genes Two growth conditions, three biological replicates of each condition. Each sample hybridised in a two-channel hybridization against Paracoccus denitrificans genomic DNA as the comparator/reference, which also acted as a control for spot quality. Cu-concentration 13 uM (Cu-H) versus 0.5 uM Cu (Cu-L) in anaerobic growth conditions.

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Transcriptome analysis of Paracoccus denitrificans during Cu-limitation

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Transcriptome analysis of Paracoccus denitrificans during aerobic and anaerobic respiration (denitrification)

Project description:Transcriptional profiling of Paracoccus denitrificans PD1222 wild type incubated in continuous culture (continuous culture (CSTR)) in minimal media with aerobic or anaerobic conditions. The goal was to define the core respiratory genes.

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RNA-seq data of Paracoccus denitrificans grown under different N2O levels

Project description:We report here the RNA seq results of sRNA enriched Paracoccus denitrificans grown under three different N2O levels (high N2O reffered to as CuL/ low N2O reffered to as CuH/ Low N2O aerobic reffered to as CuH O2)

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Impact of Electric Potential on the Transcriptome of Paracoccus denitrificans in denitrification Conditions

Project description:This study aimed to evaluate the transcriptomic response of Paracoccus denitrificans PD1222 exposed to an applied electric potential of +200 mV under aerobic denitrification conditions. RNA-seq analysis was conducted on five biological samples: three independent replicates under 200 mV and two control samples at 0 mV. Differential gene expression changes revealed genes associated with amino acid metabolism, carbohydrate metabolism, membrane transport, and energy metabolism.

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RNA-seq of Paracoccus denitrificans Pd1222 and a ramB deletion mutant during growth on succinate and acetate

Project description:The alphaproteobacterium Paracoccus denitrificans Pd1222 has two different, yet functionally redundant acetyl-CoA assimilation routes: the Ethylmalonyl-CoA pathway (EMCP) and the glyoxylate cycle (GC). The EMPC and the GC each tightly control the balance between catabolism and anabolism by shifting flux away from the oxidation of acetyl-CoA in the tricarboxylic acid (TCA) cycle towards biomass formation. RamB (encoded by Pden_1365), a member of the ScfR family, is a transcriptional regulator in P. denitrificans Pd1222 that controls expression of GC. To analyze the role of RamB in the coordinated regulation of metabolic plasticity, we analyzed the global transcriptome of P. denitrificans Pd1222, as well as the ramB-deletion strain during mid-exponential growth phase on defined minimal medium with acetate or succinate as carbon sources.

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Project description:Paracoccus denitrificans Genome sequencing

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