Project description:Illumina HiSeq technology was used to generate mRNA profiles from Pisolithus microcarpus basidiocarp compartments. Unconsolidated, young, and mature peridioles, as well as internal and free spores from three basidiocaps were harvested and used for RNA extraction. Paired-end reads of 100 bp were generated and aligned to Pisolithus microcarpus (http://genome.jgi.doe.gov/Pismi1/Pismi1.home.html) reference transcripts using CLC Genomics Workbench 7.
Project description:Illumina technology was used to generate mRNA profiles of three Pisolithus microcarpus monokaryon (M057, M091, and M120) and one dikaryon (D002) strains. Total RNAs was extracted from ectomycorrhizal (ECM) tissues and free-living mycelium (FLM) using a CTAB and lithium chloride (LiCl)-based method.TruSeq mRNA Stranded libraries were constructed and 2 x 125 bp sequenced using Illumina HiSeq 2500 at the Genotoul sequencing facilities (Toulouse, France).Raw reads were trimmed for low quality (quality score 0.05), Illumina adapters and sequences shorter than 15 nucleotides and aligned to the P. microcarpus 441 v. 2 primary scaffolds reference transcripts available at the JGI database (https://mycocosm.jgi.doe.gov) using CLC Genomics Workbench 21.0.3.
Project description:Illumina HiSeq technology was used to generate mRNA profiles from in vitro Eucalyptus grandis roots interacting with two different Pisolithus microcarpus strains (SI-9 and SI-12) and under two different CO2 concentrations (400 and 650 ppm) . Control roots or ectomycorrhizal root tips were harvested after 1 month and used for RNA extraction. Paired-end (2X150bp) reads were generated and aligned to Eucalyptus grandis transcripts (http://www.phytozome.net/; primarytranscripts only) using CLC Genomics Workbench 6.
