Arabidopsis thaliana
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA371597 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR5235490_1.fastq.gz | Fastqsanger.gz | |||
| SRR5235490_2.fastq.gz | Fastqsanger.gz | |||
| SRR5235491_1.fastq.gz | Fastqsanger.gz | |||
| SRR5235491_2.fastq.gz | Fastqsanger.gz | |||
| SRR5235492_1.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 16 |
Similar Datasets
Project description:The CLAVATA3/ESR-RELATED (CLE) peptide hormones are required for numerous plant growth and developmental processes. However, little is known regarding the function and working mechanism of the CLEs in the anther. Here, using RNA in situ hybridization analyses, we identified 7 CLE genes that are specifically expressed in the tapetum and microsporocytes in the anther, and the dominant-negative mutant plants of each of these genes exhibited significantly reduced anther size, pollen number, and abnormal pollen wall formation. Further transcriptomic and proteomic studies on cle19, DN-CLE19, and CLE19-OX mutant lines revealed that CLE19 affected the expression of more than 1,000 genes at the RNA level and 595 at the protein level, including genes involved in pollen coat and pollen exine formation, lipid metabolism, pollen germination, and hormone metabolism processes. Phenotypic analyses of mutants of the CLE19 downstream genes GRP20, ACOS5 and MEE48 revealed that the formation of pollen exine was affected in these mutants, confirming that these genes function downstream of CLE19 in the regulation of pollen wall formation. These findings demonstrate the function and downstream genes of CLE19 and redundant genes, providing insights into working pathways of the peptide hormones in pollen development.
2017-09-15 | GSE94607 | GEO
Project description:Analysis of sol2 mutant. SOL2 protein is a receptor-like kinase; involved in CLE peptide signalling pathway. Results provide insignt into; the role of SOL2 in CLE signalling. Experiment Overall Design: GeneChip analyses were performed twice for comparison of gene expression in sol2 mutant and wild type plants.
2008-10-20 | E-GEOD-11119 | biostudies-arrayexpress
Project description:Proteomic analyses of pollen coat from Arabidopsis thaliana, Arabidopsis lyrata and Brassica oleracea. Doughty 2022.
2022-12-07 | MSV000090853 | MassIVE
Project description:Plant hormones and small secretory peptides often function as environmental stress mediators. Some recent reports indicate that small secretory peptides, such as CLAVATA3/EMBRYO SURROUNDING REGION-RELATED (CLE), also function as mediators of environmental stimuli. CLE2 is induced in roots by light depriviation. Plants without functional CLE2 showed a chlorosis phenotype when grown under shade. Here, we identified specific genes downstream of CLE2 in roots and shoots with transformed Arabidopsis plants.
2020-12-31 | GSE149015 | GEO
Project description:To investigate the function of previously cloned novel cysteine-rich peptide EaF82 which biochemical properties are similar to the Rapid Alkalinization Factor (RALF), we overexpressed EaF82 gene in Arabidopsis and found defects only in the pollen development. To further determine the affected genes and biological pathways, we compared the transcriptome profilings of two independent Arabidopsis EaF82 overexpressing lines (TA and TB) with that of the vector control line (C). The RNAseq analysis results revealed decreased expressions of five endogenous clade IV AtRALFs and genes responsible for cell wall modifications and pollen maturation, supporting the observed pollen abortion.
2021-04-06 | GSE171459 | GEO
Project description:Analysis of sol2 mutant. SOL2 protein is a receptor-like kinase involved in CLE peptide signalling pathway. Results provide insignt into the role of SOL2 in CLE signalling. Keywords: gene expression array-based (RNA / in situ oligonucleotide)
2008-07-31 | GSE11119 | GEO
Project description:Cryptic Last Exons (CLEs) are unannotated terminal exons that arise from intronic regions and their inclusion results in shorter mRNA that can be translated into truncated proteins. CLE-mRNAs have been described predominantly in neurodegenerative disease, where they are widely interpreted as pathological and a consequence of splicing dysfunction. Whether CLE-mRNAs are expressed under physiological conditions or contribute to normal gene regulation remains unknown. Here, we show that CLEs are indeed used in normal cellular events. Large scale analysis of public RNA sequencing datasets revealed thousands of CLE mRNAs expressed in wild-type tissues. While CLE sequences were not conserved, CLE usage repeatedly occurred in homologous genes across species. This indicates a selection for CLE usage in specific genes and a conservation at the level of gene architecture rather than primary sequence. CLE-mRNAs display defined spatio-temporal expression patterns across development where they are associated with ribosomes. Using a CLE-mRNA previously described as abundant in ALS models (ephA4b-CLE), we demonstrate that, although initially described as pathogenic, it is temporally expressed in a tissue specific manner, translated, and essential for correct retinal ganglion cell axon growth and connectivity. Together, these findings redefine cryptic last exons as regulated transcript elements that contribute to cellular processes instead of splicing errors only relevant to disease settings.
2026-02-10 | GSE319120 | GEO
Project description:Plant hormones and small secretory peptides often function as environmental stress mediators. Some recent reports indicate that small secretory peptides, such as CLAVATA3/EMBRYO SURROUNDING REGION-RELATED (CLE), also function as mediators of environmental stimuli. CLE３ is induced in roots by defense elicitor treatment. Plants without functional CLE3 showed compromized defense gene responses in shoots when plant roots were treated with NaSA. Here, we identified specific genes downstream of CLE3 in roots and shoots with transformed Arabidopsis plants.
2021-09-01 | GSE176064 | GEO
Project description:Pollen is the male gametophyte of land plants. Proper development and maturation of pollen is necessary for the successful reproduction of seed plants. This process involves sophisticated coordination between sporophytic and gametophytic tissues in anthers. To advance the mechanistic studies of anther development, additional players need to be discovered for a comprehensive understanding of the underlying regulatory network. Here we show that the Arabidopsis dual specificity tyrosine phophorylated and regulated kinase (DRYK), AtYAK1, is essential for development of rosette leaves and the male but not female gametophyte in Arabidopsis. Arabidopsis mutant plants carrying a mutation in AtYAK1 produce developmentally stalled microspores, likely because of the defects in the two consecutive mitosis steps in the post-meiotic maturation process of pollen. The mutation of AtYAK1 has a significant effect on gene expression programs in developing pollen. Transcritpome analysis of atyak1 revealed downstream genes in families of protein kinases, transporters and transcription factors, which potentially contribute to pollen development. This study represents the first molecular characterization of DYRK in the plant kingdom. Our results also imply that the regulation of cytokinesis by DYRKs is evolutionally conserved in fungus, fruit fly, animals and plants.
2015-12-31 | GSE15971 | GEO
Project description:Differentiation processes in the primary root meristem are controlled by several signaling pathways that are regulated by phytohormones or by secreted peptides. Long term maintenance of an active root meristem requires that the generation of new root meristem cells and the loss of cells from the meristem due to differentiation is precisely coordinated. Via phenotypic and large scale transcriptome analyses of mutants, we show that the signalling peptide CLE40 and the receptor proteins CLV2 and CRN act in two genetically separable pathways that antagonistically regulate cell differentiation in the proximal root meristem. CLE40 inhibits cell differentiation throughout the primary root meristem by controlling genes with roles in abscisic acid, auxin and cytokinin signalling. CRN and CLV2 jointly control target genes that promote cell differentiation specifically in the transition zone of the proximal root meristem. While CRN and CLV2 are not acting in the CLE40 signaling pathway under normal growth conditions, both proteins are required when the levels of CLE40 or related CLE peptides increase. We show here that two antagonistically acting pathways controlling root meristem differentiation can be activated by the same peptide in a dosage dependent manner. 3 replicates reference(Col-0), clv2-gabi, cle40-2; 2 replicates crn-3
2014-10-02 | E-GEOD-59167 | biostudies-arrayexpress