Project description:The illumina NovaSeq6000 platform was utilized to generate mRNA profiles of Pinus armandii seednuts obtained in August and September. Total RNA was extracted from seed kernels from each month separately and pooled into three biological replicates for each condition. The concentration and purity of RNA (OD260/280 and OD260/230 ratios) were measured using a NanoPhotometer spectrophotometer. RNA concentration was examined for accurate quantification using a Qubit 2.0 Fluorometer, and then RNA integrity was accurately examined using an Agilent 2100 bioanalyzer.
