Project description:The Antarctic krill provides central ecosystems services to the Southern Ocean grazing on autotroph and heterotoph diet and constituting the dominant food source for higher trophic levels. Moreover, E. superba's extensive equipment with biomacromolecule hydrolysing enzymes represents a largely untapped resource for applied purposes. The proteome compendium of krill provides a valuable basis for future studies on krill biology (e.g., metabolism, development, migration behaviour), for krill's contribution to organic matter turnover in the Southern Ocean, as well as for multilevel biotechnological prospecting
Project description:The Antarctic krill provides central ecosystems services to the Southern Ocean grazing on autotroph and heterotoph diet and constituting the dominant food source for higher trophic levels. Moreover, E. superba's extensive equipment with biomacromolecule hydrolysing enzymes represents a largely untapped resource for applied purposes. The proteome compendium of krill provides a valuable basis for future studies on krill biology (e.g., metabolism, development, migration behaviour), for krill's contribution to organic matter turnover in the Southern Ocean, as well as for multilevel biotechnological prospecting.
Project description:The Antarctic krill provides central ecosystems services to the Southern Ocean grazing on autotroph and heterotoph diet and constituting the dominant food source for higher trophic levels. Moreover, E. superba's extensive equipment with biomacromolecule hydrolysing enzymes represents a largely untapped resource for applied purposes. The proteome compendium of krill provides a valuable basis for future studies on krill biology (e.g., metabolism, development, migration behaviour), for krill's contribution to organic matter turnover in the Southern Ocean, as well as for multilevel biotechnological prospecting.
Project description:The Antarctic krill provides central ecosystems services to the Southern Ocean grazing on autotroph and heterotoph diet and constituting the dominant food source for higher trophic levels. Moreover, E. superba's extensive equipment with biomacromolecule hydrolysing enzymes represents a largely untapped resource for applied purposes. The proteome compendium of krill provides a valuable basis for future studies on krill biology (e.g., metabolism, development, migration behaviour), for krill's contribution to organic matter turnover in the Southern Ocean, as well as for multilevel biotechnological prospecting.
Project description:The Antarctic krill provides central ecosystems services to the Southern Ocean grazing on autotroph and heterotoph diet and constituting the dominant food source for higher trophic levels. Moreover, E. superba's extensive equipment with biomacromolecule hydrolysing enzymes represents a largely untapped resource for applied purposes. The proteome compendium of krill provides a valuable basis for future studies on krill biology (e.g., metabolism, development, migration behaviour), for krill's contribution to organic matter turnover in the Southern Ocean, as well as for multilevel biotechnological prospecting.
Project description:Iron and light are typically recognized as major limiting factors controlling phytoplankton growth in the Southern Ocean. Recent field-based evidence suggests, however, that manganese concentrations in this region can be low enough to impact phytoplankton physiology and primary productivity. Our study examined the interactive influence of combined iron and manganese deprivation on protein expression and photophysiology in Phaeocystis antarctica, a key Antarctic phytoplankter, and provide taxon-specific proteomic evidence that natural Southern Ocean Phaeocystis populations regularly experience stress due to combined low manganese and iron availability. In culture, combined low iron and manganese induced large scale changes in the Phaeocystis proteome and resulted in reorganization of key components of the photosynthetic apparatus; these differences were largely distinct from those arising from changes in irradiance. These results implicate manganese availability as an important driver of Southern Ocean productivity and demonstrate the utility of peptide mass spectrometry as a tool for mapping of manganese contributions to HNLC conditions in this region.
Project description:Marine dissolved organic matter from Southern Ocean. Samples were analyzed by LC-MS/MS in positive and negative electrospray ionization mode using DDA (Orbitrap Eclipse).
Project description:Fragilariopsis cylindrus is an abundant diatom species in the Southern Ocean, where low iron and manganese availability constrain microbial growth and biogeochemical cycles. The molecular mechanisms used by polar diatoms including F. cylindrus to cope with trace metal limitations remain largely unexplored. Here we present phenotypic characterizations and high resolution proteomics profiles of F. cylindrus grown under controlled iron (low, medium, high) and manganese (low, high) conditions that reflect those observed in the Southern Ocean. Using data-independent acquisition mass spectrometry, we quantified over 8,000 unique proteins with high reproducibility. We captured diverse metabolic responses related to photosynthesis, elemental transport and intracellular trafficking, and protein synthesis. We show that several canonical iron stress response proteins (e.g., phytotransferrin) are consistently identified under low iron conditions, and identify additional useful iron and manganese stress biomarkers that could be explored in field measurements. Our data also support the notion that one flavodoxin copy in F. cylindrus is iron responsive and one is not. This dataset is a valuable resource for understanding trace metal physiology in polar diatoms, and provides a way for connecting molecular responses with expressed phenotypes and biogeochemical activity in the ocean.