Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Soft Hydrogels Support Differentiation of Induced Pluripotent Stem Cells toward Mesenchymal Stromal Cells

ABSTRACT: Soft Hydrogels Support Differentiation of Induced Pluripotent Stem Cells toward Mesenchymal Stromal Cells

PROVIDER: PRJNA375872 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Soft Hydrogels Support Differentiation of Induced Pluripotent Stem Cells toward Mesenchymal Stromal Cells [expression]

Project description:Induced pluripotent stem cells (iPSCs) can be differentiated toward mesenchymal stromal cells (MSCs), but at least on epigenetic level this transition remains incomplete with the current culture conditions. Hydrogels provide a more physiologic three-dimensional environment for in vitro cell culture than conventional tissue culture plastic (TCP). In this study, we followed the hypothesis that growth and differentiation of primary MSCs and of iPSC-derived MSCs (iMSCs) can be enhanced on hydrogels. To this end, we used a hydrogel made of human platelet lysate (hPL). MSCs were effectively cultured on and inside hPL-gel and demonstrated more structured deposition of extracellular matrix (ECM) components than TCP. Furthermore, hPL-gel supported differentiation of iPSCs toward MSCs. Unexpectedly, the differentiation process seemed to be hardly affected by the substrate: iMSCs generated either on TCP or hPL-gel did not reveal differences in morphology, immunophenotype, or differentiation potential. Moreover, global gene expression and DNA-methylation profiles were almost identical in iMSCs generated on TCP or hPL-gel. Our results indicate that matrix elasticity is less crucial for directed lineage-specific differentiation toward MSCs than expected.

2018-03-13 | GSE95060 | GEO

Soft Hydrogels Support Differentiation of Induced Pluripotent Stem Cells toward Mesenchymal Stromal Cells [methylation]

2018-03-13 | GSE95058 | GEO

Soft Hydrogels Support Differentiation of Induced Pluripotent Stem Cells toward Mesenchymal Stromal Cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-03-13 | GSE95061 | GEO

RNA-seq of 4T1 mouse breast cancer cells cultured on soft vs stiff polyacrylamide hydrogels for 24 hours

Project description:The tumour microenvironment is a critical element involved in tumour progression and responsiveness to therapies. Using functionalized tunable stiffness hydrogel, mimicking the mechanical properties of healthy and tumour tissues, we explore how the stiffness of the microenvironment can influence cancer cells by generating RNA-seq transcriptional profiles of 4T1 mouse breast cancer cells cultured on soft vs stiff polyacrylamide hydrogels for 24 hours.

2024-12-30 | E-MTAB-11487 | biostudies-arrayexpress

D2.0R mouse breast cancer cells cultured on stiff or soft ECM hydrogels

Project description:D2.0R mouse breast cancer cells cultured on stiff or soft ECM hydrogels

| PRJNA784565 | ENA

RNA level differences between neurons in soft and stiff environments

Project description:Hippocampal rat neurons have been cultured on very soft (100 Pa) and stiff (10 kPa) hydrogels for 7 days. On DIV7, the RNA has been extracted and sequenced. The goal of this experiment is to understand why neurons mature more quickly on soft gels compared to stiff gels.

2023-12-01 | E-MTAB-11368 | biostudies-arrayexpress

siPiezo1 or scRNA immortalised MSC cell line (Y201) cultured on soft hydrogels

Project description:Mechanosensitive ion channels have emerged as fundamental proteins in sensing extracellular matrix (ECM) mechanics. Among those, Piezo1 has been proposed as a key mechanosensor in cells. However, whether and how Piezo1 senses time-dependent ECM mechanical properties (i.e., viscoelasticity) remains unknown. To address this question, we combined an immortalised mesenchymal stem cell (MSC) line with adjustable Piezo1 expression with soft (0.4 kPa) and stiff (25 kPa) viscoelastic hydrogels with independently tuneable Young’s modulus and stress relaxation. Here, we propose that Piezo1 is an important sensor of stiffness at this range (25kPa) consistent with the molecular clutch model. By performing RNA sequencing (RNA-seq), we identified the transcriptomic phenotype of MSCs response to matrix viscoelasticity and Piezo1 activity, highlighting gene signatures that drive MSCs mechanobiology at this elasticity scale.

2025-08-20 | GSE288465 | GEO

siPiezo1 or scRNA immortalised MSC cell line (Y201) cultured on soft hydrogels

Project description:siPiezo1 or scRNA immortalised MSC cell line (Y201) cultured on soft hydrogels

| PRJNA1217810 | ENA

Homo sapiens

Project description:Soft Hydrogels Support Differentiation of Induced Pluripotent Stem Cells toward Mesenchymal Stromal Cells [expression]

| PRJNA375874 | ENA

Homo sapiens

Project description:Soft Hydrogels Support Differentiation of Induced Pluripotent Stem Cells toward Mesenchymal Stromal Cells [methylation]

| PRJNA375873 | ENA