Project description:Recent studies indicated that the differentiation tendency of pluripotent stem cells (PSCs) was affected by a certain small molecule treatment. We found the combination of small molecules that bringed out the differentiation potentials of PSCs, and defined such state of PSC as CTraS. Then, we used microarrays to detail the global programme of gene expression reflecting the effect of CTraS, and carefully compared the characteristics of iPSCs, CTraS-iPSCs, embryoid bodies (EBs), and their derived neurospheres (NSs) based on their expression profiles .
Project description:We sequenced embryoid bodies at various time points following induction of pre-mesendoderm cells (PreME) towards primordial germ cell-like cell (PGCLC) fate
Project description:Protocol to differentiate iPSC control lines into astrocytes. We performed the RNA sequencing of cells at different time point: embryoid bodies ; neurospheres at passage 1 ; neurospheres at passage 2 and differentiated astrocytes (iPast) for the 201B7 iPSC and iPast stage for WD39.
Project description:Recent reports have emphasized the pitfalls of iPSC technology including the potential for immunogenicity of transplanted cells. It is serious safety-related concern for iPSC-based cell therapy. However, preclinical data supporting the safety and efficacy of iPSCs are also accumulating. To address the concern of immunogenicity of ESCs/iPSCs or ESCs/iPSCs-derived neurospheres, global gene expression profiles were compared between undifferentiated mouse ESCs (EB3 line), mouse iPSCs (38C2 line), and ESC/iPSC-derived neurosphere and mouse primary culture of neurosphere obtained from fetal mouse ganglionic eminence. Mouse adult sklin fibroblast was used as a control. We used affymetrix microarrays to compare the global gene expression of neurospheres prepared several origins. Keywords: Expression profiling by array RNA extracted from neurospheres was hybridized to Affymetrix microarrays.
