Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA377198 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Inherited Genetic Variation and Predisposition to Testicular Germ Cell Tumor
| PRJNA377197 | ENA
Project description:Testicular germ cell tumors and their histological subgroups
| PRJNA90499 | ENA
Project description:Genome-Wide Association Study of Testicular Germ Cell Tumors
| PRJNA376226 | ENA
Project description:Post Genome-wide Association Studies in Testicular Germ Cell Tumors
| PRJNA382005 | ENA
Project description:Genomic Screening of Testicular Germ Cell Tumors from Monozygotic Twins
| PRJNA265096 | ENA
Project description:Minimum regions of imbalance involved in testicular germ cell tumours
| PRJNA97681 | ENA
Project description:Kynureninase is a member of a large family of catalytically diverse but structurally homologous pyridoxal 5'-phosphate (PLP) dependent enzymes known as the aspartate aminotransferase superfamily or alpha-family. The Homo sapiens and other eukaryotic constitutive kynureninases preferentially catalyze the hydrolytic cleavage of 3-hydroxy-l-kynurenine to produce 3-hydroxyanthranilate and l-alanine, while l-kynurenine is the substrate of many prokaryotic inducible kynureninases. The human enzyme was cloned with an N-terminal hexahistidine tag, expressed, and purified from a bacterial expression system using Ni metal ion affinity chromatography. Kinetic characterization of the recombinant enzyme reveals classic Michaelis-Menten behavior, with a Km of 28.3 +/- 1.9 microM and a specific activity of 1.75 micromol min-1 mg-1 for 3-hydroxy-dl-kynurenine. Crystals of recombinant kynureninase that diffracted to 2.0 A were obtained, and the atomic structure of the PLP-bound holoenzyme was determined by molecular replacement using the Pseudomonas fluorescens kynureninase structure (PDB entry 1qz9) as the phasing model. A structural superposition with the P. fluorescens kynureninase revealed that these two structures resemble the "open" and "closed" conformations of aspartate aminotransferase. The comparison illustrates the dynamic nature of these proteins' small domains and reveals a role for Arg-434 similar to its role in other AAT alpha-family members. Docking of 3-hydroxy-l-kynurenine into the human kynureninase active site suggests that Asn-333 and His-102 are involved in substrate binding and molecular discrimination between inducible and constitutive kynureninase substrates.
| S-EPMC2531291 | biostudies-literature
Project description:RNA extracted at 240min. Samples are rRNA depleted. Expression measurement of Homo sapiens genes.
2019-07-31 | GSE114179 | GEO
Project description:In response to signals from the embryonic testis, germ cell intrinsic factor NANOS2 coordinates a sex-specific transcriptional program necessary for differentiation of pluripotent-like primordial germ cells toward a unipotent spermatogonial stem cell fate. Emerging evidence indicates that genetic risk factors contribute to testicular germ cell tumor initiation by disrupting sex-specific differentiation. Here, using a mouse model of spontaneous testicular teratomas, we report that a subpopulation of germ cells failing to express NANOS2 is enriched for developmental phenotypes required for tumorigenesis. We demonstrate that only in the absence of NANOS2 do germ cells have a transcriptional profile enriched for Myc signaling and primed pluripotency and transform into embryonal carcinoma cells. We conclude that NANOS2 is the nexus through which many genetic risk factors exert their influence on tumor susceptibility. We propose that NODAL signaling, which is present in the developing testis, drives germ cell transformation in the absence of sex specification.
2020-09-03 | GSE157362 | GEO
Project description:We sequenced embryoid bodies at various time points following induction of pre-mesendoderm cells (PreME) towards primordial germ cell-like cell (PGCLC) fate
2023-05-05 | E-MTAB-11283 | biostudies-arrayexpress