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Staphylococcus aureus

ABSTRACT: Staphylococcus aureus comparative genomics project for Drug Resistance Evolution

PROVIDER: PRJNA37747 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR016122.fastq.gz	Fastqsanger.gz
	SRR016123.fastq.gz	Fastqsanger.gz
	SRR016124.fastq.gz	Fastqsanger.gz
	SRR016125.fastq.gz	Fastqsanger.gz
	SRR016126.fastq.gz	Fastqsanger.gz

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Staphylococcus aureus

Project description:Staphylococcus aureus comparative genomics project

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Project description:To determine if significant genomic changes are associated with the development of vancomycin intermediate Staphylococcus aureus, genomic DNA microarrays were performed to compare the initial vancomycin susceptible Staphylococcus aureus (VSSA) and a related vancomycin intermediate Staphylococcus aureus (VISA) isolate from five unique patients (five isolate pairs). Keywords: comparative genomic hybridization

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Project description:To study the effect of Radix Paeoniae Rubra decoction on tolerance of Staphylococcus aureus.The effect of Radix Paeoniae Rubra on the resistance of Staphylococcus aureus to oxacillin sodium was studied by millipore dilution method in this experiment.At the same time ,conducted on transcriptome analysis of Staphylococcus aureus related genes in Radix Paeoniae Rubra.And to detect the expression level of related genes of Staphylococcus aureus under the action of Radix Paeoniae Rubra by PCR technology.The tolerance of Staphylococcus aureus was decreased obviously when the concentration of Radix Paeoniae Rubra decoction was above 1mg/ml.The effect of Radix Paeoniae Rubra decoction on the expression of tolerance genes femB,pvL and gluM when the concentration of Radix Paeoniae Rubra decoction was above 4mg/ml.When rhe concentration of Radix Paeoniae Rubra is more than 1mg/ml,it can effectively reduce the resistance of Staphylococcus aureus to oxacillin sodium.The reason may be due to the effect of Radix Paeoniae Rubra on the resistance gene of Staphylococcus aureus.

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