Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mus musculus

ABSTRACT: H3K36me3 occupancy profiling by high throughput sequencing from control(APCmin) and APCmin Setd2IEC-/- mice intestinal cells [ChIP-seq]

PROVIDER: PRJNA377862 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR5311170.fastq.gz	Fastqsanger.gz
	SRR5311168.fastq.gz	Fastqsanger.gz
	SRR5311169.fastq.gz	Fastqsanger.gz

Items per page:

1 - 3 of 3

Similar Datasets

Intestinal mRNA profiles of 2-month old control (APCmin) and APCmin; Setd2IEC-/- mice generated by deep sequencing

Project description:Setd2 catalyzes trimethylation of lysine 36 on histone H3. H3K36me3 is deposited mainly in the gene body and has recently been demonstrated to play a role in regulating transcriptional elongation and alternative splicing. We conduct deep sequencing in 2-month old control (APCmin) and APCmin; Setd2IEC-/- mice intestinal cells to understand the splicing events regulated by Setd2.

2017-09-01 | GSE95663 | GEO

Ablation of Lgr5-positive cells in intestinal polyps from ApcMin;Lgr5DTR mice

Project description:We performed RNAseq on intestinal polyps from diptheria toxin-treated ApcMin;Lgr5DTR mice to investigate the effect of an acute selective pressure on stem cell populations in intestinal lesions. Lgr5+ cells in the ApcMin;Lgr5DTR mice were ablated with a single intraperitoneal dose of diphtheria toxin in saline (50 μg/kg), and samples were collected after 24 hours and after 5 days. Untreated ApcMin mice were used as control. Intestinal polyps were excised and collected for RNA sequencing.

2022-06-30 | E-MTAB-10470 | biostudies-arrayexpress

Mus musculus

Project description:Intestinal mRNA profiles of 2-month old control (APCmin) and APCmin Setd2IEC-/- mice generated by deep sequencing

| PRJNA377861 | ENA

RHOA inactivation enhances Wnt signaling and promotes colorectal cancer

Project description:Microarray transcriptomic analysis of formalin-fixed, paraffin-embedded small intestinal tumors from control (Apcmin/+;Vil-Cre-/-;RhoADN/-) and DN-RhoA (Apcmin/+;Vil-CreTG/-;RhoADN/-) mice.

2014-11-21 | E-MTAB-2981 | biostudies-arrayexpress

MACROD2 haploinsufficiency promotes chromosome instability and growth of intestinal tumors

Project description:RNA-Seq data from intestinal tumors of ApcMin/+/Macrod2-/-,ApcMin/+/Macrod2-/+ and ApcMin/+/Macrod2+/+ mice (6 tumors per group)

2018-06-05 | GSE93514 | GEO

The ZMYND8-regulated Mevalonate Pathway Endows YAP-High Intestinal Cancer with Metabolic Vulnerability

Project description:To elucidate the molecular basis by which ZMYND8 influences intestinal stemness and tumorigenesis, we performed RNA-seq to analyze gene expression in the crypts of 2-month-old Apcmin/+ and Apcmin/+; Zmynd8IEC–/– mice. Next, we performed chromatin immunoprecipitation (ChIP) sequencing (ChIP-Seq) to characterize the genomic distribution of ZMYND8 and SREBP2 in HCT116 WT and SREBP2-KO cells under sterol-depleted conditions.

2021-04-21 | GSE151804 | GEO

Expression Data from 12 week old APCmin/+ and littermate matched Wildtype B6 mouse Terminal Ileum (TI)

Project description:APCmin/+ mice develop spontaneous gastrointestinal polyposis due to a dominantly inhereited germline loss-of-function mutation in the tumor suppressor adenomatous polyposis coli (APC). Changes in intestinal immune activity have been documented to occur prior to the development of fulminate polyposis. Such changes are thought to contribute to disease development. We used microarrays to describe the changing intestinal transcriptional landscape in APCmin/+ mice. Whole transcriptome profiling from polypotic and nonpolypotic intestinal sections of APC/min+ mice were examined in the early stages of disease, and compared to normal intestinal sections from littermate matched wildtype B6 mice. Nonpolypotic (wildtype and APCmin/+) and Polypotic (APCmin/+) sections of terminal ileum were identified by visual inspection, and subsequently selected for RNA isolation and hydridzation to Affymetrix Mouse Genome 430A 2.0 Arrays. Interference from bacterial RNA was selected against using a probeset enriched in oligos extending into 3’-poly-A tails.

2013-08-20 | E-GEOD-49970 | biostudies-arrayexpress

RNA-seq analyses of STAT1-delta IEC ApcMin (n=3) and STAT1-flox/flox ApcMin (n=3) mice

Project description:The interferon-inducible transcription factor STAT1 is a tumor suppressor in various malignancies. We investigated STAT1 functions in intestinal tumorigenesis of ApcMin mice. Surprisingly, loss of STAT1 in intestinal epithelial cells (STAT1ΔIEC) interfered with ApcMin induced intestinal tumor formation and tumor progression. RNASeq data demonstrated reduced expression of Indoleamine-2,3-dioxygenase-1 (IDO1) in STAT1ΔIEC ApcMin tumors. IDO1 is implicated in synthesis of kynurenine, a metabolite that induces ß-Catenin nuclear localisation and suppresses anti-tumor immune responses.

2020-04-05 | E-MTAB-5083 | biostudies-arrayexpress

The epigenetic landscape of human colorectal cancer

Project description:We characterized the epigenetic landscape of human colorectal cancer (CRC). To this extent, we performed gene expression profiling using high throughput sequencing (RNA-seq) and genome wide binding/occupancy profiling (ChIP-seq) for histone modifications correlated to transcriptional activity, enhancers, elongation and repression (H3K4me3, H3K4me1, H3K27Ac, H3K36me3, H3K27me3) in patient-derived organoids (PDOs), and in normal and tumoral primary colon tissues. We also generated ChIP-seq data for transcription factors YAP/TAZ in human CRC PDOs.

2021-02-23 | E-MTAB-8416 | biostudies-arrayexpress

The epigenetic landscape of human colorectal cancer

2021-03-10 | E-MTAB-8448 | biostudies-arrayexpress