Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Helicoverpa zea

ABSTRACT: Helicoverpa zea Genome sequencing and assembly

PROVIDER: PRJNA378438 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	SRR5332814_1.fastq.gz	Fastqsanger.gz
	SRR5332814_2.fastq.gz	Fastqsanger.gz
	SRR5332816_1.fastq.gz	Fastqsanger.gz
	SRR5332816_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 27

Similar Datasets

Transcriptome sequencing of and microarray development for a Helicoverpa zea cell line to investigate in vitro insect cell-baculovirus interactions

2012-03-01 | GSE34418 | GEO

Helicoverpa zea

Project description:Helicoverpa zea alternate pseudohaplotype genome sequencing

| PRJNA807637 | ENA

Helicoverpa zea

Project description:Helicoverpa zea principal pseudohaplotype genome sequencing

| PRJNA807638 | ENA

Genome scale analysis of host-virus mRNA differential expression in Helicoverpa zea insect cells infected by H. armigera baculovirus from early till late infection

Project description:A custom microarray, based on deep transcriptome sequencing ((GEO accession number: GSE34418), was used to simultaneously investigate expression of over 24,000 Helicoverpa zea insect transcripts and 134 H. armigera nucleopolyhedrovirus (HearNPV) genes throughout the infection process at 0, 12, 24 and 48 hours post infection

2013-03-01 | GSE44184 | GEO

Helicoverpa zea

Project description:Helicoverpa zea overview

| PRJNA83915 | ENA

Helicoverpa zea

Project description:Helicoverpa zea Raw sequence reads

| PRJNA825115 | ENA

Helicoverpa zea

Project description:Helicoverpa zea Raw sequence reads

| PRJNA599999 | ENA

Helicoverpa zea

Project description:Helicoverpa zea Transcriptome or Gene expression

| PRJNA259919 | ENA

Helicoverpa zea

Project description:Helicoverpa zea Transcriptome or Gene expression

| PRJNA222356 | ENA

Transcriptome sequencing of and microarray development for a Helicoverpa zea cell line to investigate in vitro insect cell-baculovirus interactions

Project description:The Helicoverpa armigera single-capsid nucleopolyhedrovirus (HaSNPV) can be propagated using H. zea insect cell cultures, for use as a biopesticide against Heliothine agricultural pests.This study sequenced, assembled and functionally annotated 29,586 transcript sequences from cultured H. zea cells using Illumina 100 bps and paired-end transcriptome sequencing (RNA-seq). From these sequences, a genome-scale microarray platform was constructed and validated for effective expression analysis of H. zea genes. This array also included probes for all HaSNPV genes, thereby allowing virus and host gene changes to be monitored simultaneously. A 4x180,000 SurePrint Agilent expression array (Agilent, Santa Clara, CA) was employed so that a high number of probes can be included to test all 29,586 assembled H. zea sequences and to eventually select a best probe for each transcript. Two biological replicates for uninfected H. zea cells and two other biological replicates for infected samples at 18 hours post infection were analyzed. Six Agilent 60-mer oligonucleotide probes were designed by eArray (Agilent) for each transcript, in which each orientation had three probes randomly distributed across the sequences. Probes that had potential cross-hybridization (Xhyb) were removed. The final probe set for H. zea sequences included 153,583 probes. In addition, probes for all 135 H.armigera single-capsid nucleopolyhedrovirus (HaSNPV) genes were added to investigate host-virus interaction in culture.

2012-03-01 | E-GEOD-34418 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data