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Mus musculus

ABSTRACT: 2.5 dpc female HPG axis RNASeq of Kdm4a wildtype and knockout mice

PROVIDER: PRJNA379376 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5349400.fastq.gz	Fastqsanger.gz
	SRR5349410.fastq.gz	Fastqsanger.gz
	SRR5349420.fastq.gz	Fastqsanger.gz
	SRR5349401.fastq.gz	Fastqsanger.gz
	SRR5349411.fastq.gz	Fastqsanger.gz

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2.5 dpc female HPG axis RNASeq of Kdm4a wildtype and knockout mice

Project description:Purpose: We performed RNA-Seq on 2.5dpc mouse hypothalamus, pituitary, ovary and uterus of 8-10 week old littermate female control and Kdm4a knockout mice. Upon observing for the extent of altered genes of physiological relevance to observed female infertility phenotype, this would help us narrow down the most relevant tissue to perform genome wide ChipSeq binding profiles for Kdm4a, H3K4me3 and H3K9me3. This dataset would present likely target genes under direct control of Kdm4a.

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Transcriptome of Chinese sturgeon (Acipenser sinensis) from the hypothalamic-pituitary-gonad axis in two sex development stages

Project description:Here, the HPG axis transcriptomes of A. sinensis were sequenced using Illumina HiSeq 2000 and 4000 platforms. A total of 271.19 Gb filtered data from 45 samples belonging to 15 individuals (six individuals in stage I, six males and three females in stage II) were obtained, and 74.50% of 121,952 assembled unigenes were annotated to the non-redundant (NR), Swiss-Prot, Kyoto Encyclopedia of Genes and Genomes (KEGG), Cluster of Orthologous Groups of proteins (COG), InterPro, Gene Ontology (GO) and NT databases. Additionally, 78,770 unigenes contained complete open reading frames (ORFs). A total of 51,570 simple sequence repeats (SSRs) were detected, and the number of differentially expressed genes (DEGs) from 1,531 to 34,439 in 18 pairwise comparisons were identified for HPG samples of male and female A. sinensis in two stages. We reported the first integrated transcriptome data from HPG samples of male and female A. sinensis in stage I and II of gonadal development. These data offer valuable resources for research into reproductive regulation and the HPG axis interaction in A. sinensis and other sturgeons.

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Targeting KDM4A epigenetically activates tumor cell-intrinsic immunity and overcomes resistance to PD1 blockade in squamous cell carcinoma by inducing DNA replication stress

Project description:To investigate the mechanisms by which KDM4A knockout ameliorates the invasive growth and metastasis of primary mouse squamous cell carcinoma (SCC), we isolated fresh SCCs from tumor cell-specific KDM4A knockout and control mice, and then did the whole transcriptome analysis using RNA-Seq. Results showed that KDM4A knockout stimulated anti-tumor immunity.

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Total Stranded paired end RNA Seq of wildtype and knockout Kdm4a 2 cell embryos

Project description:Purpose: The goal of this study was to generate paired-end total RNA Seq transcriptomes of wildtype and Kdm4a 2 cell embryos to study the dynamics of differentially expressed repeats and transposons during early murine development Methods: 2 cell embryos cultured in KSOM EmbryoMax medium (Sigma) were subjected to the SMARTSeq Stranded Total RNASeq protocol according to the manufacturers instructions on the same day simultaneously for control and knockout embryos. Conclusions: Our study represents a detailed analysis of differentially expressed repeats and transposons in embryos lacking maternal or endogenous Kdm4a, with six biologic replicates, generated by SMARTSeq STranded paired end RNA-seq technology. The optimized data analysis workflows reported here should provide a framework for comparative investigations of expression profiles. Our results show that there is a consistent downrgulation of specific LTR families afffected in most biological replicates, by the lack of Kdm4a giving us robust statistical significance. We conclude that Kdm4a is required for proper activation of these repeat elements in conjuction with a permissive chromatin landscape.

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Identification of Kdm4a and c target genes in MLL-AF9 transformed mouse GMPs (L-GMP) [ChIP-seq]

Project description:In this study we identify genes, that are bound by Kdm4a and Kdm4c in L-GMP cells. MLL-AF9 transformed L-GMP cell lines conditional knockout for Kdm4a, b and c, were derived from leukemic mice. The cells were treated with 4-hydroxytamoxifen and chromatin was prepared from untreated cells (Ctrl) and cells treated for 96hr.

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