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Homo sapiens

ABSTRACT: UTX ChIP sequencing in non-treated MCF-7 cells

PROVIDER: PRJNA380353 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5376135_1.fastq.gz	Fastqsanger.gz
	SRR5376135_2.fastq.gz	Fastqsanger.gz
	SRR5376136_1.fastq.gz	Fastqsanger.gz
	SRR5376136_2.fastq.gz	Fastqsanger.gz
	SRR5376137_1.fastq.gz	Fastqsanger.gz

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Homo sapiens whole blood.

Project description:RNA extracted at 240min. Samples are rRNA depleted. Expression measurement of Homo sapiens genes.

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Characterizing the contrasting roles of JMJD3 and UTX histone demethylases in T cell acute lymphoblastic leukemia [GSKJ4_ChIP-seq]

Project description:T-cell acute lymphoblastic leukemia (T-ALL) is an immature hematopoietic malignancy driven mainly by oncogenic activation of NOTCH1 signaling. In this study we chemically inhibited the H3K27me3 demethylase JMJD3 using the GSKJ4 inhibitor and assayed for genome-wide changes in H3K27me3 and JMJD3 enrichment. This piece of data was further integrated to expression changes using RNA sequencing as well as ChIP-Sequencing analysis of H3K27me3 upon genomic knock-down of JMJD3 and UTX. These results, coupled to genomic analysis of primary samples for the genomic status of the UTX gene in T-ALL, helped us to identify a hitherto unknown role of JMJD3 as an oncogenice facilitator in leukemia whereas UTX seems to play a tumor suppressor role. Histone ChIP: Half to one million cells were treated with micrococcal nuclease (MNASE) to generate mononucleosomal particles and an adaptation of the Upstate ChIP protocol was used.

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