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RIP-seq and RNA-seq in wt and nono-/- mice

ABSTRACT: RIP-seq and RNA-seq in wt and nono-/- mice

PROVIDER: PRJNA383795 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5464190.fastq.gz	Fastqsanger.gz
	SRR5464210.fastq.gz	Fastqsanger.gz
	SRR5464220.fastq.gz	Fastqsanger.gz
	SRR5464230.fastq.gz	Fastqsanger.gz
	SRR5464240.fastq.gz	Fastqsanger.gz

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Diurnal liver transcriptome in wt and nono-/- mice

Project description:The liver circadian transcriptome results from the combined action of a circadian clock and feeding/fasting rhythms. Since we found that (1) the number of NONO-containing speckles increases in response to feeding, (2) NONO interacts with RNA processing factors and (3) it binds to the introns of a large number of protein coding genes, we hypothesized that NONO may contribute to the daily rhythm of hepatic gene expression. To address whether NONO contributes to rhythmic gene expression in response to feeding/fasting, diurnal liver transcriptomes were assessed in nono-/- and wt littermate mice fed a normal diet during the night time (ZT12-24). We habituated mice to a 12:12 LD cycle with food available only at lights off (ZT12-24) for one week and in the second week collected mice every 2h throughout the 24h day. Both total and nuclear RNAs were sequenced after ribosomal RNA depletion

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anti-NONO RIP seq in liver nuclei after fasting and re-feeding

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Comparative Analysis of the hippocampal transcriptome of WT and NONO KO mice

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NONO preferentially binds to exon-intron boundary and regulates alternative splicing in Rat Cardiac Fibroblasts [RIP-seq]

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Project description:siRNA knockdown of NONO mRNA followed by RNA-seq was carried out in the KELLY neuroblasotma cell line. In order to determine potential regulatory targets of NONO protein, we performed differential expression analysis. To identify the RNA targets of NONO in neuroblastoma, we carried out PAR-CLIP (Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation) using an antibody recognising NONO protein.

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