Project description:Bisphenols F (BPF) and S (BPS) are BPA structural analogs used in many marketed products as a replacement for BPA. Since sparse toxicological data are available yet for these bisphenol substitutes, our objective was to comprehensively characterize bisphenols gene targets in a human primary adipocyte model, using chronic exposure at two concentrations: a “low-dose” similar to the dose usually encountered in human biological fluids and a higher dose.
Project description:Bisphenols F (BPF) and S (BPS) are BPA structural analogs used in many marketed products as a replacement for BPA. Since sparse toxicological data are available yet for these bisphenol substitutes, our objective was to comprehensively characterize bisphenols gene targets in a human primary adipocyte model, using chronic exposure at two concentrations: a “low-dose” similar to the dose usually encountered in human biological fluids and a higher dose.
Project description:The effects of LXR stimulation by GW3965 treatment on global mRNA and miRNA expression in primary human in vitro differentiated adipocytes was investigated using microarray profiling.
Project description:Acute phase reactants serum amyloid A-1, 3 and micro RNA-135b, -449a, and -1 are induced in lungs of mice exposed to subtoxic doses of nano-titanium dioxide particles by inhalation In the present study we investigate pulmonary mRNA and miRNA profiles of mice exposed to subtoxic dose of nano-titanium dioxide particles by inhalation. We show dramatic induction of acute phase reactants, chemoattractants, immune and host defence related genes. We also demonstrate for the first time changes in miRNA profiles in the lungs in response to nanoTiO2. Keywords: Toxicology, disease state analysis, biomarkers of health effects
Project description:We compared the variations of expression of miRNA/small nucleolar RNA (snoRNA) in a series of post-Chernobyl tumors developed in patients exposed to radioactive iodines, at thyroid doses below (21 to 48 mGy) or above (0·58 to 2·5 Gy) the limit of significance of conventional epidemiology, and in a series of unexposed Ukrainian patients. We identified miRNA/snoRNA discriminating signatures of thyroid PTC etiology in function of the dose range (“Low doses” or “High doses”) or independent of the dose (“Low+High doses”), as compared to sporadic tumors. Moreover, corresponding signatures of radiation exposure were also found analysing the non-pathologic tissues adjacent to the tumors of exposed and non-exposed patients, which showed an overlap with the etiology signatures.
Project description:Transcriptional profiling of primary rat hepatocytes exposed to high and low doses of three compounds, as well as untreated cells. All samples were co-hybridized with DMSO controls.
Project description:In this study, we investigate mRNA profiles in lung of mice exposed to benzo(a)pyrene. Male mice were exposed to three doses (25, 50, and 75 mg/kg/day BaP) for 28 days and profiles were examined three days post-exposure. Our analyses reveal that BaP causes pulmonary specific cellular transformation indicative of carcinogenesis.
