Sludge metagenome
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA387347 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR6043961_1.fastq.gz | Fastqsanger.gz | |||
| SRR6043961_2.fastq.gz | Fastqsanger.gz | |||
| SRR6043962_1.fastq.gz | Fastqsanger.gz | |||
| SRR6043962_2.fastq.gz | Fastqsanger.gz | |||
| SRR6043963_1.fastq.gz | Fastqsanger.gz |
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Project description:Wastewater treatment plants use a variety of bioreactor types and configurations to remove organic matter and nutrients. Little is known regarding the effects of different configurations and within-plant immigration on microbial community dynamics. Previously, we found that the structure of ammonia-oxidizing bacterial (AOB) communities in a full-scale dispersed growth activated sludge bioreactor correlated strongly with levels of NO2- entering the reactor from an upstream trickling filter (Wells et al 2009). Here, to further examine this puzzling association, we profile within-plant microbial biogeography (spatial variation) and test the hypothesis that substantial microbial immigration occurs along a transect (raw influent, trickling filter biofilm, trickling filter effluent, and activated sludge) at the same full-scale wastewater treatment plant. AOB amoA gene abundance increased >30-fold between influent and trickling filter effluent concomitant with NO2- production, indicating unexpected growth and activity of AOB within the trickling filter. Nitrosomonas europaea was the dominant AOB phylotype in trickling filter biofilm and effluent, while a distinct ‘Nitrosomonas-like’ lineage dominated in activated sludge. Prior time series indicated that this ‘Nitrosomonas-like’ lineage was dominant when NO2- levels in the trickling filter effluent (i.e., activated sludge influent) were low, while N. europaea became dominant in the activated sludge when NO2- levels were high. This is consistent with the hypothesis that NO2- production may co-occur with biofilm sloughing, releasing N. europaea from the trickling filter into the activated sludge bioreactor. Phylogenetic microarray (PhyloChip) analyses revealed significant spatial variation in taxonomic diversity, including a large excess of methanogens in the trickling filter relative to activated sludge and attenuation of Enterobacteriaceae across the transect, and demonstrated transport of a highly diverse microbial community via the trickling filter effluent to the activated sludge bioreactor. Our results provide compelling evidence that substantial immigration between coupled process units occurs and may exert significant influence over microbial community dynamics within staged bioreactors.
2015-11-03 | GSE52079 | GEO
Project description:Manufactured nanomaterials (MNMs) are increasingly incorporated into consumer products that are disposed into sewage. In wastewater treatment, MNMs adsorb to activated sludge biomass where they may impact biological wastewater treatment performance, including nutrient removal. Here, we studied MNM effects on bacterial polyhydroxyalkanoate (PHA), specifically polyhydroxybutyrate (PHB), biosynthesis because of its importance to enhanced biological phosphorus (P) removal (EBPR). Activated sludge was sampled from an anoxic selector of a municipal wastewater treatment plant (WWTP), and PHB-containing bacteria were concentrated by density gradient centrifugation. After starvation to decrease intracellular PHB stores, bacteria were nutritionally augmented to promote PHB biosynthesis while being exposed to either MNMs (TiO2 or Ag) or to Ag salts (each at a concentration of 5 mg L-1). Cellular PHB concentration and PhyloChip community composition were analyzed. The final bacterial community composition differed from activated sludge, demonstrating that laboratory enrichment was selective. Still, PHB was synthesized to near-activated sludge levels. Ag salts altered final bacterial communities, although MNMs did not. PHB biosynthesis was diminished with Ag (salt or MNMs), indicating the potential for Ag-MNMs to physiologically impact EBPR through the effects of dissolved Ag ions on PHB producers.
2014-12-03 | GSE63781 | GEO
Project description:Manufactured nanomaterials (MNMs) are increasingly incorporated into consumer products that are disposed into sewage. In wastewater treatment, MNMs adsorb to activated sludge biomass where they may impact biological wastewater treatment performance, including nutrient removal. Here, we studied MNM effects on bacterial polyhydroxyalkanoate (PHA), specifically polyhydroxybutyrate (PHB), biosynthesis because of its importance to enhanced biological phosphorus (P) removal (EBPR). Activated sludge was sampled from an anoxic selector of a municipal wastewater treatment plant (WWTP), and PHB-containing bacteria were concentrated by density gradient centrifugation. After starvation to decrease intracellular PHB stores, bacteria were nutritionally augmented to promote PHB biosynthesis while being exposed to either MNMs (TiO2 or Ag) or to Ag salts (each at a concentration of 5 mg L-1). Cellular PHB concentration and PhyloChip community composition were analyzed. The final bacterial community composition differed from activated sludge, demonstrating that laboratory enrichment was selective. Still, PHB was synthesized to near-activated sludge levels. Ag salts altered final bacterial communities, although MNMs did not. PHB biosynthesis was diminished with Ag (salt or MNMs), indicating the potential for Ag-MNMs to physiologically impact EBPR through the effects of dissolved Ag ions on PHB producers. 18 samples; Triplicate PHB-enriched bacterial communities recovered from activated sludge were exposed to nanoparticle (TiO2 or Ag) or AgNO3 (as a silver control) or were not exposed to an nanoparticles (control) to determine if the naoparticles affected PHB production.
2014-12-03 | E-GEOD-63781 | biostudies-arrayexpress
Project description:nitrosation granular sludge microbial community diversity
| PRJNA387346 | ENA
Project description:activated granular sludge microbial community diversity
| PRJNA387345 | ENA
Project description:This study investigates the role of carbon-to-phosphorus (C/P) ratios in shaping microbial community dynamics and polyhydroxyalkanoates (PHA) production in sequencing batch reactors (SBR) fed with volatile fatty acids. Three conditions, characterized by fixed organic loading rates but varying C/P ratios (Run 1 = 170 Cmol Pmol-1; Run 2 = 235 Cmol Pmol-1; Run 3 = 400 Cmol Pmol-1), were tested to explore their impact on PHA accumulation, biomass growth, and reactor stability. Results indicate that the moderate phosphorus limitation of Run 2 achieves the best overall performance, with a PHA volumetric productivity of 2.02 g PHA L-1 d-1 and process stability. Conversely, higher C/P ratio increased the storage yield but lowered the productivity (1.55 gPHA L-1 d-1), compromising sludge settleability and reactor stability, indicating impaired microbial functionality. Full-length 16S/18S rRNA gene sequencing using PacBio technology enabled high-resolution profiling of microbial communities, revealing ecological shifts across conditions. Run 2 exhibited the highest bacterial and eukaryotic diversity, featuring multiple PHA-accumulating bacteria (mainly Sphaerotilus, Leadbetterella, and uncultured Rhodobacteraceae) and a well-structured eukaryotic community dominated by K-strategist bacterivorous protists, e.g. Rhogostoma (Rhizaria) and Vorticella (Ciliophora, Oligohymenophorea) and predatory protozoa, e.g. Tokophrya (Ciliophora, Suctoria). In contrast, Sphaerotilus dominated under suboptimal conditions and may be linked to bulking. These results suggest that eukaryotic diversity may support system stability through selective predation. Transmission electron microscopy (TEM) further confirmed the presence of intracellular PHA granules and polyphosphate reserves, reinforcing the connection between nutrient limitation and adaptive microbial strategies. Overall, these findings highlight the critical role of the C/P ratio in shaping the performance of mixed microbial cultures, demonstrating that a well-balanced nutrient supply can enhance PHA production while maintaining microbial community stability. The results contribute to optimizing the selection process for mixed microbial cultures, offering valuable insights into the impact of carbon-to-nutrient ratios in the feeding strategy.
2025-07-14 | GSE302520 | GEO
Project description:This study investigates the role of carbon-to-phosphorus (C/P) ratios in shaping microbial community dynamics and polyhydroxyalkanoates (PHA) production in sequencing batch reactors (SBR) fed with volatile fatty acids. Three conditions, characterized by fixed organic loading rates but varying C/P ratios (Run 1 = 170 Cmol Pmol-1; Run 2 = 235 Cmol Pmol-1; Run 3 = 400 Cmol Pmol-1), were tested to explore their impact on PHA accumulation, biomass growth, and reactor stability. Results indicate that the moderate phosphorus limitation of Run 2 achieves the best overall performance, with a PHA volumetric productivity of 2.02 g PHA L-1 d-1 and process stability. Conversely, higher C/P ratio increased the storage yield but lowered the productivity (1.55 gPHA L-1 d-1), compromising sludge settleability and reactor stability, indicating impaired microbial functionality. Full-length 16S/18S rRNA gene sequencing using PacBio technology enabled high-resolution profiling of microbial communities, revealing ecological shifts across conditions. Run 2 exhibited the highest bacterial and eukaryotic diversity, featuring multiple PHA-accumulating bacteria (mainly Sphaerotilus, Leadbetterella, and uncultured Rhodobacteraceae) and a well-structured eukaryotic community dominated by K-strategist bacterivorous protists, e.g. Rhogostoma (Rhizaria) and Vorticella (Ciliophora, Oligohymenophorea) and predatory protozoa, e.g. Tokophrya (Ciliophora, Suctoria). In contrast, Sphaerotilus dominated under suboptimal conditions and may be linked to bulking. These results suggest that eukaryotic diversity may support system stability through selective predation. Transmission electron microscopy (TEM) further confirmed the presence of intracellular PHA granules and polyphosphate reserves, reinforcing the connection between nutrient limitation and adaptive microbial strategies. Overall, these findings highlight the critical role of the C/P ratio in shaping the performance of mixed microbial cultures, demonstrating that a well-balanced nutrient supply can enhance PHA production while maintaining microbial community stability. The results contribute to optimizing the selection process for mixed microbial cultures, offering valuable insights into the impact of carbon-to-nutrient ratios in the feeding strategy.
2025-07-14 | GSE302519 | GEO
Project description:This study investigates the role of carbon-to-phosphorus (C/P) ratios in shaping microbial community dynamics and polyhydroxyalkanoates (PHA) production in sequencing batch reactors (SBR) fed with volatile fatty acids. Three conditions, characterized by fixed organic loading rates but varying C/P ratios (Run 1 = 170 Cmol Pmol-1; Run 2 = 235 Cmol Pmol-1; Run 3 = 400 Cmol Pmol-1), were tested to explore their impact on PHA accumulation, biomass growth, and reactor stability. Results indicate that the moderate phosphorus limitation of Run 2 achieves the best overall performance, with a PHA volumetric productivity of 2.02 g PHA L-1 d-1 and process stability. Conversely, higher C/P ratio increased the storage yield but lowered the productivity (1.55 gPHA L-1 d-1), compromising sludge settleability and reactor stability, indicating impaired microbial functionality. Full-length 16S/18S rRNA gene sequencing using PacBio technology enabled high-resolution profiling of microbial communities, revealing ecological shifts across conditions. Run 2 exhibited the highest bacterial and eukaryotic diversity, featuring multiple PHA-accumulating bacteria (mainly Sphaerotilus, Leadbetterella, and uncultured Rhodobacteraceae) and a well-structured eukaryotic community dominated by K-strategist bacterivorous protists, e.g. Rhogostoma (Rhizaria) and Vorticella (Ciliophora, Oligohymenophorea) and predatory protozoa, e.g. Tokophrya (Ciliophora, Suctoria). In contrast, Sphaerotilus dominated under suboptimal conditions and may be linked to bulking. These results suggest that eukaryotic diversity may support system stability through selective predation. Transmission electron microscopy (TEM) further confirmed the presence of intracellular PHA granules and polyphosphate reserves, reinforcing the connection between nutrient limitation and adaptive microbial strategies. Overall, these findings highlight the critical role of the C/P ratio in shaping the performance of mixed microbial cultures, demonstrating that a well-balanced nutrient supply can enhance PHA production while maintaining microbial community stability. The results contribute to optimizing the selection process for mixed microbial cultures, offering valuable insights into the impact of carbon-to-nutrient ratios in the feeding strategy.
2025-07-14 | GSE302521 | GEO