Project description:The aim of this project is to determine the differential expression of genes between monocytes infiltrating HCC nodules in the absence versus in the presence of LECT2
Project description:The aim of this project is to determine the differential expression of genes between poorly differntiated HCC tumor compared to differntiated tumor arising in HCC/LECT2-KO mice
Project description:CD8+ T cells are master effectors of anti-tumor immunity and their presence at tumor sites correlates with favorable outcomes. However, metabolic constraints imposed by the tumormicroenvironment can dampen their ability to control tumor progression. We will analyze the transcriptional profile of CD8+ T cells infiltrating murine tumors during progression in a pre-cinical model pancreatic ductal adenocarcinoma (PDA).
Project description:We performed scRNAseq analysis of murine monocytes cultured from bone marrow isolates of WT and TRAM knockout mice. WT and TRAM knockout monocytes were cultured in vitro for 5 days in the presence or absence of low dose LPS (100 pg/ml).
Project description:LECT2 is a liver derived cytokine and involved in many pathologic conditions, especially in immune regulation. To better understand cellular and molecular mechanisms of LECT2 in immune response, mouse peritoneal macrophages were isolated from mouse peritonaeum. After treatment with 0, 0.5 or 5 μg/ml of LECT2 for 3.5 or 48 hours, gene expression profile in mouse peritoneal macrophages was measured using gene expression array. Results showed that LECT2 treatment led to the enhancement of cytokines secretion and phagocytosis in peritoneal macrophages.
Project description:Comparing the gene expression profile of human NPC undergoing neural differentiation in the presence and absence of NeuroAid MLC901.
Project description:Ayu, Plecoglossus altivelis, is an economically important amphidromous fish species cultured in East Asia. LECT2 is a liver derived cytokine and involved in many pathologic conditions, especially in immune regulation. To understand cellular and molecular mechanisms of LECT2 in immune response of ayu, macrophages were isolated from ayu head kidney. After treatment with 0, 0.5 or 5 μg/ml of LECT2 for 3.5 hour, gene expression profile in ayu macrophages was measured using gene expression array. Results showed that LECT2 treatment led to the altered expression of immune related genes in ayu macrophages.
