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Staphylococcus aureus subsp. aureus USA300_FPR3757

ABSTRACT: The Staphylococcus aureus α-Acetolactate Synthase ALS Confers Resistance to Nitrosative Stress

PROVIDER: PRJNA388956 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5639113.fastq.gz	Fastqsanger.gz
	SRR5639114.fastq.gz	Fastqsanger.gz
	SRR5639115.fastq.gz	Fastqsanger.gz
	SRR5639116.fastq.gz	Fastqsanger.gz

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The Staphylococcus aureus α-Acetolactate Synthase ALS Confers Resistance to Nitrosative Stress

Project description:Staphylococcus aureus is a worldwide pathogen that colonizes the human nasal cavity and is a major cause of respiratory and cutaneous infections. In the nasal cavity, S. aureus thrives with high concentrations of nitric oxide (NO) produced by the innate immune effectors and has available for growth slow metabolizing free hexoses, such as galactose. Here, we have used deep sequencing transcriptomic analysis (RNA-Seq) and 1H-NMR to uncover how S. aureus grown on galactose, a major carbon source present in the nasopharynx, survives the deleterious action of nitric oxide. We observed that, like on glucose, S. aureus withstands high concentrations of NO when using galactose. However, most likely this is achieved through a distinct metabolism that relies on the increased production of amino acids, such as glutamate, threonine and branched-chain amino acids. Moreover, we found that under these conditions the α-acetolactate synthase (ALS) enzyme, which converts pyruvate into α-acetolactate, plays a role in the resistance of S. aureus to NO. However, the role of ALS is not restricted to galactose but also extends to cells growing on glucose. The results suggest that ALS prevents intracellular acidification, promoting the production of branched-chain amino acids and activation of the TCA cycle. We show that ALS contributes to the successful infection of murine macrophages. Furthermore, ALS is also shown to contribute to the resistance of S. aureus to beta-lactam antibiotics such as methicillin and oxacillin.

2017-06-02 | GSE99563 | GEO

Global expression profiling to study the effect of triazolopyrimidine herbicide treatment on Arabidopsis thaliana

Project description:Using whole genome microarray (Affymetrix ATH1) we studied the transcriptional response of Arabidopsis thaliana to triazolopyrimidine (FirstRate) herbicde that inhibits acetolactate synthase (ALS) enzyme and thus disrupts branched chain amino acid biosynthesis. A number of genes related to amino acid, protein metabolism, growth, regulatory networks, respiratory pathways, stress, defense and secondary metabolism were altered. Keywords: Acetolactate synthase (ALS) inhibiting herbicide stress response

2009-07-22 | GSE8926 | GEO

Global expression profiling to study the effect of imidazolinone herbicide treatment on Arabidopsis thaliana

Project description:Using whole genome microarray (Affymetrix ATH1) we studied the transcriptional response of Arabidopsis thaliana to imidazolinone (Arsenal) herbicde that inhibits acetolactate synthase (ALS) enzyme and thus disrupts branched chain amino acid biosynthesis. A number of genes related to amino acid, protein metabolism, growth, regulatory networks, respiratory pathways, stress, defense and secondary metabolism were altered. Keywords: Acetolactate synthase (ALS) inhibiting herbicide stress response

2009-07-22 | GSE8925 | GEO

Global expression profiling to study the effect of primisulfuron herbicide treatment on Arabidopsis thaliana

Project description:Using whole genome microarray (Affymetrix ATH1) we studied the transcriptional response of Arabidopsis thaliana to primisulfuron (Beacon) herbicde that inhibits acetolactate synthase (ALS) enzyme and thus disrupts branmched chain amino acid biosynthesis. A number of genes related to amino acid, protein metabolism, growth, regulatory networks, respiratory pathways, stress, defense and secondary metabolism were altered. Keywords: Acetolactate synthase (ALS) inhibiting herbicide stress response

2009-07-22 | GSE8913 | GEO

Global expression profiling to study the effect of sulfometuron methyl herbicide treatment on Arabidopsis thaliana

Project description:Using whole genome microarray (Affymetrix ATH1) we studied the transcriptional response of Arabidopsis thaliana to sulfometuron methyl (oust XP) herbicde that inhibits acetolactate synthase (ALS) enzyme and thus disrupts branmched chain amino acid biosynthesis. A number of genes related to amino acid, protein metabolism, growth, regulatory networks, respiratory pathways, stress, defense and secondary metabolism were altered. Keywords: Acetolactate synthase (ALS) inhibiting herbicide stress response

2009-07-22 | GSE8912 | GEO

Staphylococcus aureus subsp. aureus USA300_FPR3757

Project description:Staphylococcus aureus subsp. aureus USA300_FPR3757 Raw sequence reads

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The Staphylococcus aureus nitric oxide synthase (saNOS) modulates respiratory metabolism and cell physiology

Project description:Nitric oxide synthase (NOS) enzymes produce nitric oxide (NO), a highly reactive free radical capable of interacting with multiple cellular targets. Although saNOS contributes to Staphylococcus aureus virulence, as well as protection against exogenous oxidative stress and antimicrobials, the current mechanism behind these phenotypes is unknown. Here we report a previously-undescribed role for saNOS in modulating S. aureus physiology. When grown aerobically, endogenous reactive oxygen species (ROS) and superoxide levels were elevated in a S. aureus nos mutant. NO has been shown to slow respiration in other organisms, and likewise, comparison of respiratory dehydrogenase activity and membrane potential in wild-type, nos mutant, and complement strains suggested that saNOS-derived NO limits S. aureus aerobic respiration. Multiple transcriptional and metabolic changes were also observed in a S. aureus nos mutant, as assessed by RNAseq and targeted metabolomics analyses, respectively. Specifically, expression of genes associated with oxidative and nitrosative stress responses, anaerobic and lactate metabolism, and cytochrome biosynthesis and assembly were increased in the nos mutant relative to wild-type. Metabolites utilized to produce reducing equivalents by the right arm of the TCA cycle were depleted in a nos mutant (citrate and α-ketoglutarate), whereas fumarate and malate levels were increased relative to wild-type and complement strains. A significant reduction in lactate levels was also observed in the nos mutant. Collectively, these results support a model in which the absence of saNOS results in increased respiration and ROS accumulation, which may signal the cells to switch to an alternative lactate-based respiratory metabolism.

2017-07-10 | GSE77400 | GEO

Staphylococcus aureus subsp. aureus USA300_FPR3757

Project description:A methicillin resistant strain of Staphylococcus aureus

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Global transcriptional profiling of Staphylococcus aureus treated with rhein

Project description:Staphylococcus aureus is one of the most important pathogens in humans and animals, multiply resistant strains are increasingly widespread, new agents are needed for the treatment of S. aureus. Rhein, a natural plant product, has potential antimicrobial activity against Staphylococcus aureus. We employed Affymetrix Staphylococcus aureus GeneChipsTM arrays to investigate the global transcriptional profiling of Staphylococcus aureus ATCC25923 treated with rhein. Results provided insight into mechanisms involved in rhein - Staphylococcus aureus interactions. Keywords: rhein response

2007-06-15 | GSE8135 | GEO

Project description:Bromus japonicus acetolactate synthase (ALS) mRNA, complete cds

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