Drosophila melanogaster
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA389132 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR5643483_1.fastq.gz | Fastqsanger.gz | |||
| SRR5643483_2.fastq.gz | Fastqsanger.gz | |||
| SRR5643484_1.fastq.gz | Fastqsanger.gz | |||
| SRR5643484_2.fastq.gz | Fastqsanger.gz | |||
| SRR5643485_1.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 12 |
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Project description:DUOX, a member of the NADPH oxidase family, acts as the first line of host defense against enteric pathogen by producing microbicidal reactive oxygen species. Despite the extensive research conducted, the elucidation of the DUOX regulatory mechanism remains incomplete. Here, by taking advantages of the Drosophila genetic tools, we showed that enteric infection acts as a ‘pro-catabolic’ signaling capable of initiating metabolic reprogramming of enterocytes toward lipid catabolism through activation of complex signaling cascades involving TNF receptor-associated factor 3, AMP-activated protein kinase, Warts kinase of the Hippo pathway, and ATG1 kinase of autophagy. We further showed that inflammation-modulated metabolic reprogramming is necessary for cellular NADPH homeostasis, which is essential for sustained DUOX activity and host survival during enteric infection. The discovery of signaling cascades governing inflammation-induced metabolic reprogramming will provide a novel perspective on physiology and pathophysiology of immune-metabolic interactions in microbe-laden gut epithelia.
2017-09-28 | GSE99642 | GEO
Project description:<p>Viral studies of Drosophila melanogaster typically involve virus injection with a small needle, causing post-injury a wounding/wound healing response, in addition to the effects of viral infection. However, the metabolic response to the needle injury is understudied, and many viral investigations neglect potential effects of this response. Furthermore, the wMel strain of the endosymbiont bacterium Wolbachia pipientis provides anti-viral protection in Drosophila. Here we used NMR-based metabolomics to characterise the acute wounding response in Drosophila and the relationship between wound healing and the Wolbachia strain wMel. The most notable response to wounding was found on the initial day of injury and lessened with time in both uninfected and Wolbachia infected flies. Metabolic changes in injured flies revealed evidence of inflammation, Warburg-like metabolism and the melanisation immune response as a response to wounding. In addition, at five days post injury Wolbachia infected injured flies were metabolically more similar to the uninjured flies than uninfected injured flies were at the same time point, indicating a positive interaction between Wolbachia infection and wound healing. This study is the first metabolomic characterisation of the wound response in Drosophila and its findings are crucial to the metabolic interpretation of viral experiments in Drosophila in both past and future studies.</p>
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Project description:Circular RNAs (circRNAs) are widely expressed in eukaryotes. However, only a subset has been functionally characterized. We have identified and validated a collection of circular RNAs in Drosophila melanogaster. We show that the circular RNA circ_ATP8B is induced by viral infection and that depletion of circ_ATP8B, but not its linear sibling, compromises viral infection both in cultured cells and in vivo. In addition, our analyses reveal that circ_ATP8B is enriched in the fly gut and that gut-specific depletion of circ_ATP8B attenuates viral replication in an oral infection model. Furthermore, we find circ_ATP8B-depletion resulted in increased levels of reactive oxygen species (ROS) and enhanced expression of Duox (Dual oxidase), which produces ROS. Genetic and pharmacological manipulation of circ_ATP8B-depleted flies that reduce ROS levels rescue the viral replication defects elicited by circ_ATP8B depletion. Notably, circ_ATP8B and Duox associate with each other, and that expression of various versions of circ_ATP8B that are competent in binding Duox, but not a mutant circ_ATP8B that is incapable of binding Duox, restores physiological levels of ROS in circ_ATP8B-depleted cells. Lastly, our data show that Gaq, a subunit of G protein required for optimal Duox activity, acts downstream of circ_ATP8B to regulate Duox activity. We conclude that circ_ATP8B regulates anti-viral immunity by modulating Duox-dependent ROS production.
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Project description:Circular RNAs (circRNAs) are widely expressed in eukaryotes. However, only a subset has been functionally characterized. We have identified and validated a collection of circular RNAs in Drosophila melanogaster. We show that the circular RNA circ_ATP8B is induced by viral infection and that depletion of circ_ATP8B, but not its linear sibling, compromises viral infection both in cultured cells and in vivo. In addition, our analyses reveal that circ_ATP8B is enriched in the fly gut and that gut-specific depletion of circ_ATP8B attenuates viral replication in an oral infection model. Furthermore, we find circ_ATP8B-depletion resulted in increased levels of reactive oxygen species (ROS) and enhanced expression of Duox (Dual oxidase), which produces ROS. Genetic and pharmacological manipulation of circ_ATP8B-depleted flies that reduce ROS levels rescue the viral replication defects elicited by circ_ATP8B depletion. Notably, circ_ATP8B and Duox associate with each other, and that expression of various versions of circ_ATP8B that are competent in binding Duox, but not a mutant circ_ATP8B that is incapable of binding Duox, restores physiological levels of ROS in circ_ATP8B-depleted cells. Lastly, our data show that Gaq, a subunit of G protein required for optimal Duox activity, acts downstream of circ_ATP8B to regulate Duox activity. We conclude that circ_ATP8B regulates anti-viral immunity by modulating Duox-dependent ROS production.
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