Project description:To identify the mechanisms of the adaptation to terrestrial ecosystems, an RNA-seq based transcriptome analysis was conducted on a desiccation resistant cyanobacterium, Nostoc sp. MG11.
Project description:To investigate the function of All0854, we constructed the all0854 deletion mutant Mall0854, in which all0854 was knocked out by CRISPER-cpf1. We then performed gene expression profiling analysis using data obtained from RNA-seq of wide type Nostoc sp. PCC 7120 and Mall0854.
Project description:The ability of microbes to adapt to highly dynamic environments depends on subtle post-transcriptional regulatory mechanisms. The interaction between antisense RNAs or small non-coding RNAs and their mRNA targets can alter their half-life, thereby playing a role in the adaptation to different physiological conditions. Here, we have used rifampicin, an antibiotic that inhibits transcription initiation, to estimate the half-life of all transcripts in Nostoc sp. PCC 7120, a heterocyst-forming cyanobacterium. The analysis of the rifampicin time-series under two different growth conditions has revealed new layers of post-transcriptional regulation that may include transcriptional interference events (collisions between two elongating RNA polymerases).