Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Ruminococcus bromii

ABSTRACT: Ruminococcus bromii Genome sequencing and assembly

PROVIDER: PRJNA390910 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	SRR5712035.fastq.gz	Fastqsanger.gz
	SRR5712036.fastq.gz	Fastqsanger.gz
	SRR5712037.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

Ruminococcus bromii

Project description:Ruminococcus bromii overview

| PRJNA39189 | ENA

Ruminococcus bromii L2-63

Project description:Ruminococcus bromii L2-63 genome sequencing project

| PRJNA39153 | ENA

Sas20 is a highly flexible starch-binding protein in the Ruminococcus bromii cell-surface amylosome

Project description:Ruminococcus bromii is a keystone species in the human gut that has the rare ability to degrade dietary resistant starch (RS). This bacterium secretes a suite of starch-active proteins that work together within larger complexes called amylosomes that allow R. bromii to adhere to and degrade RS. Sas20 is one of the more abundant proteins assembled within amylosomes, but little could be predicted about its molecular features based upon amino acid sequence. Here, we perform a structure-function analysis of Sas20 which features two discrete starch-binding domains separated by a flexible linker. Sas20 domain 1 has an N-terminal β-sandwich followed by a cluster of α-helices and captures the non-reducing end of maltooligosaccharides between these structural features. The crystal structure of a close homolog of Sas20 domain 2 revealed a unique bilobed starch-binding groove that targets the helical 1,4-linked glycan chains found in amorphous regions of amylopectin and crystalline regions of amylose within starch granules. Affinity PAGE and isothermal titration calorimetry demonstrate both domains bind maltoheptaose and soluble starch with relatively high affinity (Kd 20 M) but exhibit limited or no binding to cyclodextrins. Small angle x-ray scattering analysis of the individual and combined domains support that these structures are highly flexible, which may allow the protein to adopt conformations that enhance its starch-targeting efficiency.

2022-06-09 | PXD032013 | Pride

Ruminococcus bromii TSDC17.2-1.1

Project description:Strain Ruminococcus bromii TSDC17.2-1.1 (species Ruminococcus bromii) was isolated from the fecal microbiota of a USA female at time point zero (bacterial isolates were sequenced from this donor on day 0 and 49). The species name was assigned by genome clustering.

| PRJEB3777 | ENA

Ruminococcus bromii TSDC10.2-1.1

Project description:Strain Ruminococcus bromii TSDC10.2-1.1 (species Ruminococcus bromii) was isolated from the fecal microbiota of a USA female at time point zero (bacterial isolates were sequenced from this donor on day 0 and 42). The species name was assigned by genome clustering.

| PRJEB3618 | ENA

Ruminococcus bromii TSDC10.1-1.1

Project description:Strain Ruminococcus bromii TSDC10.1-1.1 (species Ruminococcus bromii) was isolated from the fecal microbiota of a USA female at time point zero (bacterial isolates were sequenced from this donor on day 0 and 42). The species name was assigned by genome clustering.

| PRJEB3572 | ENA

Ruminococcus bromii TSDC17.2-1.2

Project description:Strain Ruminococcus bromii TSDC17.2-1.2 (species Ruminococcus bromii) was isolated from the fecal microbiota of a USA female at time point zero (bacterial isolates were sequenced from this donor on day 0 and 49). The species name was assigned by genome clustering.

| PRJEB3778 | ENA

Ruminococcus bromii TSDC17.2-1.3

Project description:Strain Ruminococcus bromii TSDC17.2-1.3 (species Ruminococcus bromii) was isolated from the fecal microbiota of a USA female at time point zero (bacterial isolates were sequenced from this donor on day 0 and 49). The species name was assigned by genome clustering.

| PRJEB3779 | ENA

RNA-seq of Ruminococcus bromii L2-63 and Ruminococcus gnavus ATCC 29149 grown in mono- or co-culture with different carbon sources (glucose, soluble or resistant starch)

Project description:The goal was to assess the impact of carbon source and cross-feeding on transcription profile of both bacteria. R. gnavus was grown in monoculture with glucose (Glc). R. bromii was grown in monoculture with soluble (SS) or resistant (RS) starch. R. bromii and R. gnavus were co-cultured with SS or RS. Total RNA was extracted from a culture sample taken at mid- to late exponential phase of growth. rRNA was depleted and mRNA sequenced. 3 biological replicates were prepared for each condition.

2019-07-31 | E-MTAB-7138 | biostudies-arrayexpress

Expression profiling of Ruminococcus flavefaciens FD-1 with growth on cellulose or cellobiose

Project description:This study compares growth of Ruminococcus flavefaciens FD-1 with cellulose or cellobiose as the carbohydrate substrate. Ruminococcus flavefaciens is a predominant cellulolytic rumen bacterium, which forms a multi-enzyme cellulosome complex that could play an integral role in the ability of this bacterium to degrade plant cell wall polysaccharides. Identifying the major enzyme types involved in plant cell wall degradation is essential for gaining a better understanding of the cellulolytic capabilities of this organism as well as highlighting potential enzymes for application to improvement of livestock nutrition and for conversion of cellulosic biomass to liquid fuels. These results show that the growth substrate drives expression of enzymes predicted to be involved in carbohydrate metabolism as well as expression and assembly of key cellulosomal enzyme components. 1 species (Ruminococcus flavefaciens FD_1), 2 conditions (cellulose, cellobiose), 4 biological replicates. Direct design with biological dye swap.

2010-05-16 | E-GEOD-15916 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data