Project description:<p>In the US, CRC incidence has declined with uptake in colonoscopy for early detection and removal of polyps, the precursor lesion, can stop a cancer from developing but in spite of this, CRC remains the second leading cause of cancer death in the United States. One third of people who undergo screening colonoscopy will have adenomatous polyps, but less than 5% of the time are these polyps presumed to go on to develop into cancer. Why does one polyp develop into cancer while another one that looks very similar does not. In this proposal we will identify what molecular genetic changes in the genome, in the mRNA expression and genetic methylation patterns will distinguish a polyp that has already transformed into cancer from one that has not.</p>
Project description:Identification of molecular features that predict the malignant potential of a polyp is a major clinical step in individualizing polyp patient management. Why does one polyp develop into cancer while another does not? Our first aim was to validate our original findings on differences between polyps based on transformation in an expanded and independent cohort of patients. Our next aim was to identify the molecular events that define the transition point of polyps to cancer based on the aggressiveness of the polyp (polyp outcome phenotype) to develop a cancer risk prediction model for polyps.
Project description:Identification of molecular features that predict the malignant potential of a polyp is a major clinical step in individualizing polyp patient management. Why does one polyp develop into cancer while another does not? Our first aim was to validate our original findings on differences between polyps based on transformation in an expanded and independent cohort of patients. Our next aim was to identify the molecular events that define the transition point of polyps to cancer based on the aggressiveness of the polyp (polyp outcome phenotype) to develop a cancer risk prediction model for polyps.
Project description:Detached Arabidopsis leaves can regenerate adventitious roots, providing a platform to study de novo root regeneration (DNRR). We performed time-lapse RNA-seq within 12 h to reveal transcriptional changes in response to early signals in DNRR.
Project description:Here we investigate the transcriptional landscapes of nasal polyp IgD+ (naïve-like) B cells, nasal polyp ASC, and blood naïve B cells using RNA-seq. These data found that nasal polypP IgD+ naïve-like B cells are activated and similar to nasal polyp ASC and distinct from circulating B cells in the blood.
Project description:Detached Arabidopsis leaves can regenerate adventitious roots, providing a platform to study de novo root regeneration (DNRR). We performed time-lapse RNA-seq within 5 d revealed activation of gene networks in cell fate transition.