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Gallus gallus

ABSTRACT: Deep Sequencing Analysis of miRNAs Expression in leg muscle of Xinghua Chicken

PROVIDER: PRJNA391315 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5725950.fastq.gz	Fastqsanger.gz
	SRR5725951.fastq.gz	Fastqsanger.gz
	SRR5725942.fastq.gz	Fastqsanger.gz
	SRR5725952.fastq.gz	Fastqsanger.gz
	SRR5725943.fastq.gz	Fastqsanger.gz

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Deep Sequencing Analysis of miRNAs Expression in leg muscle of Xinghua Chicken

Project description:Purpose:We have used RNA-seq to examine of differentially expressed miRNAs in chicken leg muscle of three different development stages (11 embryo ages, 16 embryo ages, and 1 day old post hatch chick).The aims of this study are characterization of miRNAs differentially expressed in different developmental stage of chicken embryo, using RNA sequence sample. Methods: On this study we used two embryonic stage and one post hatch chick leg muscle of Xinghua chicken breed. Total RNA from E11 day embryo, E16 day embryo and 1 day post hatch chick was isolated by TRIzol following the manufacturer’s protocol (Invitrogen, CA, USA). Each stages were designed two samples, and the total samples were six (three group × two sample/group) and RNA samples of six individuals were pooled with equal amounts, and then were subjected to Illumina deep sequencing. Results: After eliminating adaptor and low-quality reads, a total of 5,302,700, 6,556,747, 5,359,793, 4,213,112, 7,112,885 and 7,469,939 clean reads were obtained in group E11 (E11.1-E11.2), group E16 (E16.1-E16.2) and group P1 (P1.1–P1.2) libraries, respectively. The clean reads were aligned to the chicken genome databases, miRBase, Rfam, RepBase and mRNA. Conclusions:To assess miRNA expression during chicken embryo skeletal muscle development, we sequenced and analyzed leg muscle at 11 day embryo age, 16 day embryo age, and 1 days post hatch.

2017-06-22 | GSE100321 | GEO

Gallus gallus

Project description:Genome-wide analysis of long non-coding RNA (lncRNA) profiles in leg muscle of Xinghua Chickens.

| PRJNA356759 | ENA

Gallus gallus

Project description:RNA-seq of RNase R treated and ribo- RNAs from leg muscle of Xinghua Chickens

| PRJNA351879 | ENA

Deep Sequencing Analysis of miRNAs Expression in Breast Muscle of Fast-Growing and Slow-Growing Broilers

Project description:To investigate the function of miRNAs in chicken growth, breast muscle tissues of the two-tail samples (highest and lowest body weight) from Recessive White Rock (WRR) and Xinghua Chickens (XH) were performed on high throughput small RNA deep sequencing. In this study, a total of 921 miRNAs were identified, including 733 known mature miRNAs and 188 novel miRNAs. There were 200, 279, 257 and 297 differentially expressed miRNAs in the contrasts of WRRh Vs. WRRl, WRRh Vs. XHh, WRRl Vs. XHl, and XHh Vs. XHl group, respectively. A total of 22 serious differentially expressed miRNAs (fold change > 2 or < 0.5; P-value < 0.05; q-value < 0.01) which also have abundant expression (read counts > 1,000) were found in our contrasts. As far as two contrasts (WRRh Vs. WRRl, and XHh Vs. XHl) are concerned, we found 80 common differentially expressed miRNAs, meanwhile 110 miRNAs were found in WRRh Vs. XHh and WRRl Vs. XHl. Furthermore, only 26 common miRNAs were identified among all of four contrasts.

2014-11-05 | GSE62971 | GEO

Deep Sequencing Analysis of miRNAs Expression in Breast Muscle of Fast-Growing and Slow-Growing Broilers

2014-11-05 | E-GEOD-62971 | biostudies-arrayexpress

Gallus gallus

Project description:Deep Sequencing Analysis of miRNAs Expression in Breast Muscle of Fast-Growing and Slow-Growing Broilers

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Gallus gallus

Project description:Identification of miRNAs involved in embyonic chicken muscle development

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Gallus gallus breed:Xinghua Chicken

Project description:Gallus gallus breed:Xinghua Chicken Raw sequence reads

| PRJNA1103130 | ENA

Gallus gallus breed:Xinghua Chicken

Project description:Gallus gallus breed:Xinghua Chicken Raw sequence reads

| PRJNA1102297 | ENA

Characterization of miRNA transcriptome of skeletal muscle from broiler and layer chicken lines by deep sequencing

Project description:In the current study, we expanded our previous work to identify miRNAs implicated in the myogenesis regulation through the comparison of miRNAs transcriptome in skeletal muscle tissues between broilers and layers. To address that, we firstly performed Solexa deep sequencing to profile miRNAs expressed in chicken skeletal muscle tissues. Sequence tags analyses not only enable us to report a group of highly abundant known miRNAs expressed in skeletal muscles but most importantly to identify novel putative chicken miRNAs from skeletal muscle tissue. Further miRNA transcriptome comparison and real-time RT-PCR validation experiments revealed seveal differentially expressed miRNAs between broilers and layers.

2011-03-18 | GSE20942 | GEO

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