Project description:A transcriptome of Cluster II Frankia in nitrogen-fixing root-nodule symbiosis with the host plant, Datisca glomerata, was obtained by Illumina sequencing and mapping to the corresponding published genome (NCBI Bioproject PRJNA46257). Major metabolic pathways detected in Cluster II Frankia in symbiosis with Datisca glomerata were comparable to those described as up-regulated in the Frankia alni-Alnus glutinosa symbiosis (N Alloisio et al, MPMI 23(5):593-607, 2010): nitrogenase biosynthesis, tricarboxylic acid cycle, respiratory-chain related functions, oxidation protection, and terpenoid biosynthesis. These functions are consistent with the primary activities of Frankia in root nodules, e.g. to carry out the energetically-demanding fixation of atmospheric dinitrogen to ammonium, and to maintain internal reducing conditions. Expression of genes coding for amino-acid biosynthetic pathways, including arginine as reported previously (AM Berry et al. Funct Plant Biol 38, 645–652, 2011) was detected. A striking difference from other Frankia strains, revealed in the transcriptome of the Cluster II Frankia in symbiosis, was the expression of homologs of rhizobial nodulation genes, nodA, nodB and nodC.
Project description:We characterized the polyethylene glycol (PEG)-responding desiccome from the most geographically widespread Gram-positive nitrogen-fixing plant symbiont, i.e. Frankia alni, by next-generation proteomics.
Project description:The proteomics analysis of the nitrogen-fixin bacterium Frankia alni, the tree Alnus glutinosa (Alder), and the oomycete plant pathogen Phytophthora alni was carried out by shotgun proteomics from nodules to establish the defence mechanisms of the symbiont during an infection.
Project description:Purpose: To compare RNASeq data of Frankia inefficax EuI1c under selenite stress. Frankia cultures were grown for 4 days under 0.1 mM selenite stress or without selenite (Control). RNA-seq analysis provided insight into how the the bacteria responds to selenite stress and exhibits selenite tolerance.
Project description:Purpose: To compare RNASeq data of Frankia inefficax EuI1c in normal growth conditions to biphenyl-stressed Frankia inefficax EuI1c. Frankia cultures were grown for 3 days under one of three conditions before harvesting: 1.) 1 mM biphenyl stress with glucose as an alternative carbon and energy source, 2.) 1mM biphenyl with no alternative carbon or energy source, or 3.) media with glucose as a carbon and energy source (Control). RNA-seq analysis provided insight into how the the bacteria responds to biphenyl stress and exhibits biphenyl tolerance.
