Project description:T cells were extracted from spleens of CBLA mice and activated in vitro with CD3/28 on day 0. They were spinoculated with retroviruses overexpressing a 3xFLAG-gene of interest on day 1. On day 4, BFP-positive (infected) cells were FACS sorted and measured by ChIPmentation. For input ChIP controls, see E-MTAB-6276
Project description:Plasma cells from spleen were FACS sorted using Blmp1 and CD138 expression and gene expression compared to naïve B cells, FACS sorted using B220 and IgD
