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Bos taurus

ABSTRACT: Reduced representation bisulfite sequencing (RRBS) data from cattle monocytes, fibroblasts and sperm cells

PROVIDER: PRJNA396902 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5890540_1.fastq.gz	Fastqsanger.gz
	SRR5890540_2.fastq.gz	Fastqsanger.gz
	SRR5890541_1.fastq.gz	Fastqsanger.gz
	SRR5890541_2.fastq.gz	Fastqsanger.gz
	SRR5890542_1.fastq.gz	Fastqsanger.gz

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Embryonic heat conditioning induces paternal heredity of immunological cross- tolerance: coordinative role of CpG DNA methylation and miR-200a regulation [RNA-seq]

Project description:The first-generation embryos underwent inovo heat conditioning (EHC) and the offspring were subjected to an intracranial lipopolysaccharide (LPS) challenge, demonstrating immune resilience in second generation which is mediated through the paternal DNA methylation pattern. DNA was extracted from the sperm and reduced representation bisulfite sequencing (RRBS) was performed

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High-dose folic acid supplementation alters the human sperm methylome and is influenced by the MTHFR C677T polymorphism

Project description:Dietary folate is a major source of methyl groups required for DNA methylation, an epigenetic modification that is actively maintained and remodelled during spermatogenesis. While high dose folic acid supplementation (up to ten times the daily recommended dose) has been shown to improve sperm parameters in infertile men, the effects of supplementation on the sperm epigenome are unknown. To assess the impact of six months of high dose folic acid supplementation on the sperm epigenome, we studied 30 men with idiopathic infertility. Blood folate concentrations increased significantly after supplementation with no significant improvements in sperm parameters. Methylation levels of the differentially methylated regions of several imprinted loci (H19, DLK1/GTL2, MEST, SNRPN, PLAGL1, KCNQ1OT1) were normal both before and after supplementation. Reduced representation bisulfite sequencing (RRBS) revealed a significant global loss of methylation across different regions of the sperm genome. The most marked loss of DNA methylation was found in sperm from patients homozygous for the methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism, a common polymorphism in a key enzyme required for folate metabolism. RRBS analysis also showed that most of the differentially methylated tiles were located in DNA repeats, low CpG density and intergenic regions. Ingenuity Pathway Analysis revealed that methylation of promoter regions was altered in several genes involved in cancer and neurobehavioral disorders including CBFA2T3, PTPN6, COL18A1, ALDH2, UBE4B, ERBB2, GABRB3, CNTNAP4 and NIPA1. Our data reveal alterations of the human sperm epigenome associated with high dose folic acid supplementation, effects that were exacerbated by a common polymorphism in MTHFR. Reduced representation bisulfite sequencing of 28 human sperm samples before and after 6 month of high dose folic acid supplementation.

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Project description:We used Reduced Representation Bisulfite Sequencing (RRBS) to comprehensively investigate the impact of chronic, low-dose Cd exposure on mice spermatozoa DNA methylation. Adult male C57BL/J6 mice were provided water with or without cadmium chloride. We purified genomic DNA from sperm samples collected after nine weeks of treatment. We found 1,788 differentially methylated sites present at regulatory regions in sperm of mice exposed to chronic, low-dose of Cd compared to vehicle (control) mice. Our results present a comprehensive analysis of the sperm methylome in response to chronic Cd exposure

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