Project description:The primary aim of this study is
- to explore the usefulness of re-examination and retroflexion on adenoma miss rate (AMR) in the proximal colon.
Other aims include to explore the data below when re-examination or retroflexion is used.
* Adenoma detection rate, ADR
* Polyp miss rate, PMR
* Polyp detection rate, PDR
* Withdrawal time, WT
Project description:Multiple pathways prevent DNA replication from occurring more than once per cell cycle. These pathways block re-replication by strictly controlling the activity of pre-replication complexes, which assemble at specific sites in the genome called origins. Here we show that mutations in the homologous histone 3 lysine 27 (H3K27) monomethyltransferases, ARABIDOPSIS TRITHORAX-RELATED PROTEIN5 (ATXR5) and ATXR6, lead to re-replication of specific genomic locations. The vast majority of these locations correspond to transposons and other repetitive and silent elements of the Arabidopsis genome. These sites also correspond to high levels of H3K27 monomethylation, and mutation of the catalytic SET domain is sufficient to cause the re-replication defect. Mutation of ATXR5 and ATXR6 also causes upregulation of transposon expression and has pleiotropic effects on plant development. These results uncover a novel pathway that prevents over-replication of heterochromatin in Arabidopsis. Examination of genomic DNA content in endoreduplicating nuclei in wild type and atxr atxr6 mutant plants
Project description:Introduction Reflux esophagitis (RE) is a common upper gastrointestinal disorder, and its diagnosis currently relies primarily on invasive endoscopic examination. The lack of reliable non-invasive biomarkers substantially limits early detection and large-scale screening. Saliva represents a promising biofluid for metabolomics research, as it can reflect metabolic alterations associated with upper gastrointestinal pathology. Objectives This study aimed to identify potential salivary lipid biomarkers associated with RE, and to develop a non-invasive diagnostic model using metabolomics and lipidomics. Methods Saliva samples from patients clinically diagnosed with RE and healthy controls were analyzed. The analysis included a discovery cohort (n = 144) and an independent validation cohort (n = 146). Differential metabolites were screened using the untargeted metabolomics approach of ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS), and then quantitative verification was conducted using targeted lipidomics. Multivariate statistical analysis, random forest algorithms, and receiver operating characteristic (ROC) analysis were applied. Results Untargeted metabolomics revealed significant metabolic differences between RE patients and healthy controls, with marked enrichment of sphingolipid and glycerophospholipid metabolism. Targeted lipidomics identified six consistently dysregulated salivary lipids: DAG (18:1_18:2), S-1-P, PE (P-16:0_18:1), DAG (16:0_18:2), DAG (18:1_18:1), and DAG (16:0_18:1). A multimetabolite model based on these lipids effectively distinguished RE patients from healthy controls, achieving an AUC of 99.45% in the discovery cohort and 97.17% in the validation cohort. Conclusion This study identified a salivary lipid signature associated with RE and supports the potential of this lipidomic approach as a non-invasive method to distinguish RE from healthy controls.
Project description:Strains 2-22 (S. agalactiae ST261 isolated from fish) and A909 (ST7) were grown in TH medium, at 30C and harvested at OD 0.3-0.4. Please note: ST261 and ST7 refer to MLST types commonly used in S.agalactiae as a first approach for phylogenomic relationships (MLST is based on the sequence of 7 genes).
Project description:Strains A909 (ST7 strain isolated from human) and CF01173 (ST7 strain isolated from fish) were grown in TH medium at 37C and harvested at OD 0.3-0.4. Please note: ST7 refers to MLST types commonly used in S.agalactiae as a first approach for phylogenomic relationships (MLST is based on the sequence of 7 genes).
Project description:All patients planned for an anterior resection due to rectal cancer with a total mesorectal excision are included. This is a feasibility study, thus no randomization will be performed.
Primary endpoint is clinical and pathologic examination of the specimen. Secondary end-points include clinical variables such as conversion rate, re-admission and/or re-operation due to any complication and health economy analyses.
Project description:The prevention of COVID-19 pandemic is highly complicated by the prevalence of asymptomatic and recurrent infection. Many previous immunological studies have focused on symptomatic and convalescent patients, while the immune responses in asymptomatic patients and re-detectable positive cases remain unclear. Here we comprehensively analyzed the peripheral T-cell receptor (TCR) repertoire of 54 COVID-19 patients in different courses, including asymptomatic, symptomatic, convalescent and re-detectable positive cases. We identified a set of V-J gene combinations characterizing the upward immune responses through asymptomatic and symptomatic courses. Furthermore, some of these V-J combinations could be awakened in the re-detectable positive cases, which may help predict the risk of recurrent infection. Therefore, TCR repertoire examination has the potential to strengthen the clinical surveillance and the immunotherapy development for COVID-19.
2025-10-19 | GSE186120 | GEO
Project description:Phylogenomic analyses re-examine the evolution of reinforcement and hypothesized hybrid speciation in Phlox wildflowers
Project description:Tet1 is a hydroxylase known for its role in the conversion of 5-methylcytosines (5mC) to 5-hydroxymethylcytosines (5hmC) involved in the possible active demethylation process and gene expression regulation1-5.M-BM- As somatic cell reprogramming involves the re-activation of pluripotency genes and the silencing of somatic ones6, it remains unclear whether Tet1 plays a positive or negative role in the reprogramming process. Here we show that Tet1 deficiency enhances reprogramming and its overexpression impairs reprogramming. Mechanistically, we demonstrated that Tet1 represses the early obligatory process of mesenchymal to epithelial transition (MET) during reprogramming7,8. Thus, our findings not only define a negative role for Tet1 in somatic cell reprogramming, but also suggest that the Tet enzymes regulate cell fate through distinctive mechanisms. Examination of genome DNA hmC modifications in 2 conditions: individually overexpressed Tet1CD or Tet2CD during MEF reprogramming; Examination of mRNA levels in five different conditions: individually overexpressed DR or Tet1CD or Tet1CDmut or Tet2CD or Tet2CDmut, during MEF reprogrammig.