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Homo sapiens

ABSTRACT: Differential expression of microRNAs in productively HIV-1 infected and bystander macrophages in culture.

PROVIDER: PRJNA401712 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5998750.fastq.gz	Fastqsanger.gz
	SRR5998751.fastq.gz	Fastqsanger.gz
	SRR5998752.fastq.gz	Fastqsanger.gz
	SRR5998744.fastq.gz	Fastqsanger.gz
	SRR5998745.fastq.gz	Fastqsanger.gz

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Differential expression of microRNAs in productively HIV-1 infected and bystander macrophages in culture.

Project description:Macrophages are heterogeneous immune cells with distinct origins, phenotypes, functions and tissue localization. Their susceptibility to HIV-1 is subject to variations from permissiveness to resistance, owing in part to regulatory microRNAs. Here, we used RNAseq to examine the expression of >400 microRNAs in productively infected and bystander cells of HIV-1-exposed macrophage cultures. Two micro-RNAs up regulated in bystander macrophages, miR-221 and miR-222, were identified as negative regulators of CD4 expression and CD4-mediated HIV-1 entry. Both microRNAs were enhanced by TNF-α, an inhibitor of CD4 expression. MiR-221/miR-222 inhibitors recovered HIV-1 entry in TNF-α-treated macrophages by enhancing CD4 expression, and increased HIV-1 replication and spread in macrophages by countering TNF-α-enhanced miR-221/miR-222 expression in bystander cells. In line with these findings, HIV-1-resistant intestinal myeloid cells express higher levels of miR-221 than peripheral blood monocytes. Thus, miR-221/miR-222 act as effectors of the antiviral host response activated during macrophage infection that restrict HIV-1 entry.

2017-09-11 | GSE103394 | GEO

Global mapping of the macrophage-HIV-1 transcriptome reveals that productive infection induces remodeling of the host cell DNA and chromatin

Project description:It has been proposed that macrophages could serve as long-lived compartments for HIV-1 infection under in vivo situations because these cells are resistant to the virus-mediated cytopathic effect, produce progeny virus over extended periods of time and are localized in tissues that are often less accessible by treatment. Comprehensive experimental studies are thus needed to characterize the HIV-1-induced modulation of host genes in these myeloid lineage cells. To shed light on this important issue, we performed comparative analyses of mRNA expression levels of host genes in uninfected bystander and HIV-1-infected human macrophages using an infectious reporter virus construct coupled with a large-scale RNA sequencing approach. We observed a rapid differential expression of several host factors in the productively infected macrophage population including genes regulating DNA replication factors and chromatin remodeling. A siRNA-mediated screening study to functionally identify host determinants involved in HIV-1 biology has provided new information on the virus molecular regulation in macrophages.

2021-07-21 | GSE180273 | GEO

miRNA expression profiles in productively HIV-1 infected and bystander primary human CD4+ T-cells

Project description:CD4+ T-cells are the main target of HIV-1 and several host factors can positively or negatively modulate HIV-1 infection of these cells. MiRNAs aresmall regulatory RNAs that are involved in the regulation of basic cellular functions. They are also increasingly recognized as host factors regulatingHIV-1 infection, replication and persistence. In order to identify miRNAs involved in HIV-1 infection of CD4+ T-cells, we performed globaltranscriptomic analyses of productively infected and HIV-1 exposed but non infected bystander CD4+ T-cells and compared their MIRNA profiles with uninfected cells. Theseanalyses were performed in CD4+T-cells isolated from 3 different healthy blood donors. Both miRNA and mRNA expression profiles were compared. Our results show that bystander and uninfected CD4+ T-cells do not display important differences in their miRNA expression profiles even though their respectivemRNA expression profiles were markedly different. In contrast, both mRNA and miRNA expressionprofiles from productively infected CD4+ T-cells were significantly different from those of uninfected cells. Overall, these results suggest that HIV-1infection impacts the miRNA expression profile of primary CD4+ T-cells.

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mRNA expression profiles in productively HIV-1 infected and bystander primary human CD4+ T-cells

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Productively HIV-1 infected and bystander primary human CD4+ T-cells

Project description:Productively HIV-1 infected and bystander primary human CD4+ T-cells

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Transcriptional profiling of HIV dual reporter infected Jurkat T cells using CAGE.

Project description:This study examine the transcriptional profiling of HIV dual reporter non-infected, latetnly infected and productively infected Jurkat T cells.

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miRNA expression profiles in productively HIV-1 infected and bystander primary human CD4+ T-cells

Project description:miRNA expression profiles in productively HIV-1 infected and bystander primary human CD4+ T-cells

| PRJNA1036612 | ENA

mRNA expression profiles in productively HIV-1 infected and bystander primary human CD4+ T-cells

Project description:mRNA expression profiles in productively HIV-1 infected and bystander primary human CD4+ T-cells

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Project description:Differential Gene Expression in HIV-Infected Individuals Following ART.

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Project description:Expression analysis of microRNAs in plasma of new HIV-infected cases, old HIV-infected cases and healthy controls.