Project description:Acute myocardial infarction is a leading cause of death among adults globally. Hypoxia/reoxygenation (H/R) injury plays a causal role in myocardial damage and death, whose molecular mechanisms remain largely elusive. Our present work employed rat H9C2 cardiomyocytes to establish an H/R injury model and investigated differential expression profiles following H/R treatment using RNA-seq. Normal cultured H9C2 cardiomyocytes were used as the control group.
Project description:We evaluated the profile of lncRNA and mRNA expression in control and 50μg/ml DEP treated HBE cells using the Arraystar Human LncRNA Array v3.0 array,7th generation. Our findings implicates that dysregulation of mitochondria invovled mRNAs may play important role in cytotoxicity of DEP. Examination of lncRNA and mRNA expression in control or DEP-treated HBE cells
Project description:We evaluated the profile of lncRNA and mRNA expression in control and 50μg/ml DEP treated HBE cells using the Arraystar Human LncRNA Array v3.0 array,7th generation. Our findings implicates that dysregulation of mitochondria invovled mRNAs may play important role in cytotoxicity of DEP.
Project description:LncRNA Chaer contributes to the transcriptome reprogramming during phenylephrine-induced hypertrophy in neonatal rat ventricular myocytes
Project description:Using the highly sensitive lncRNA array (Arraystar human lncRNA microarray V3), we screened the lncRNAs and mRNA abundant in shZNF717-HepG2 and shCtrl-HepG2 cell lines, and the functions of ZNF717 in liver cancer were analyzed along the following research.
Project description:To elucidate the molecular mechanism and signaling pathways that PinX1 is involved in, a Human LncRNA Array V2.0 (Arraystar, USA)-based genome wide screen of the alterations in lncRNA and mRNA expression profiles was performed in MCF-7 cells stably overexpressing PinX1.
Project description:Using the highly sensitive lncRNA array, we screened the lncRNAs abundant in the human bladder cancer and Adjacent normal bladder tissues, and the function of differentially expressed lncRNAs were analyzed by bioinformatics. The Arraystar Human lncRNA Array (8x15K, Arraystar) and whole human mRNA Array (4x44K, Arraystar) and was used to profile differentially expressed lncRNAs and genes in bladder cancer vs. normal tissues following the manufacturerâs instructions. Briefly, extracted RNA template (1mg) was reversely transcribed into cDNA and digested into fragments with endonucleases. These fragments were labeled with DNA labeling reagent and labeled cDNAs were hybridized to the microarray via incubation at 45°C and rotated at 60 rpm for 17 h. Following washing and staining, the arrays were scanned using a GeneChip Scanner3000 with GeneChip Operating Software.
Project description:reports shows that EOC cell under hypoxia condition shows high malignancy. So we use the Arraystar Human LncRNA Microarray V3.0 to verify the difference expression of LncRNA in the exosomes drived from skov-3 cells which cultured in nomor/hypoxia condition
