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Chicken gut metagenome

ABSTRACT: Runting-Stunting poultry disease overview

PROVIDER: PRJNA40799 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR027046.fastq.gz	Fastqsanger.gz
	SRR027047.fastq.gz	Fastqsanger.gz
	SRR027048.fastq.gz	Fastqsanger.gz
	SRR027049.fastq.gz	Fastqsanger.gz

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Project description:Reticuloendotheliosis virus (REV), a member of the Gammaretrovirus genus in the Retroviridae family, causes an immunosuppressive, oncogenic and runting-stunting syndrome in multiple avian hosts. To better understand the host interactions at the transcriptional level, microarray data analysis was performed in chicken embryo fibroblast (CEF) cells at 1, 3, 5, and 7 days after infection with REV. This study identified numerous differentially expressed genes that were classified into several functional groups. Significant differences were mainly observed in the expression of genes involved in the immune response, especially during the later post-infection time points. These results revealed that differentially expressed genes play important roles in the pathogenicity of REV infection. Our study is the first to use microarray analysis to investigate REV, and these findings provide insights into the underlying mechanisms of the host antiviral response and the molecular basis of viral pathogenesis.

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Project description:Marek's disease (MD), induced by Marek's disease virus (MDV), is a lymphotropic neoplastic disease and causes huge economic losses to the poultry industry. Non-coding RNAs play important regulatory roles in disease pathogenesis. To investigate host miRNA expression profile, RNA sequencing was performed in tumorous spleens (TS), spleens from the survivors (SS) without any lesion after MDV infection, and noninfected chicken spleens (NS).

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