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Identification of Human Juvenile Chondrocyte-specific factors

ABSTRACT: Identification of Human Juvenile Chondrocyte-specific factors

PROVIDER: PRJNA408287 | ENA |

REPOSITORIES: ENA

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Identification of Human Juvenile Chondrocyte-specific factors

Project description:Although regeneration of human cartilage is inherently inefficient, age is an important risk factor for Osteoarthritis (OA). Recent reports have provided compelling evidence that juvenile chondrocytes (from donors below 13 years of age) are more efficient at generating articular cartilage as compared to adult chondrocytes. However, the molecular basis for such a superior regenerative capability is not understood. In order to identify the cell-intrinsic differences between juvenile and adult cartilage, we have systematically profiled global gene expression changes between a small cohort of human neonatal/juvenile and adult chondrocytes. No such study is available for human chondrocytes although ‘young’ and ‘old’ bovine and equine cartilage have been recently profiled.

2017-09-22 | GSE104113 | GEO

mRNA expression profiles of engineered chondrocyte sheets

Project description:Cartilage injury does not naturally repair and lead to osteoarthritis. Engineered chondrocyte sheets are readily transplantable and create neocartilage in vivo. This experiment compares global mRNA profiling of adult and juvenile chondrocyte sheets.

2025-06-30 | GSE235008 | GEO

Identification of chondrocyte subsets during human development

Project description:Joint injury and osteoarthritis affect millions of people worldwide, but attempts to generate articular cartilage using adult stem/progenitor cells have been unsuccessful. We hypothesized that recapitulation of the human developmental chondrogenic program using pluripotent stem cells (PSCs) may represent a superior approach for cartilage restoration. Using laser capture microdissection followed by microarray analysis, we first defined a surface phenotype (CD146low/negCD166low/negCD73+CD44lowBMPR1B+) distinguishing the earliest cartilage committed cells (pre-chondrocytes) at 5-6 weeks of development; pellet assays confirmed these cells as functional, chondrocyte-restricted progenitors. Flow cytometry, qPCR and immunohistochemistry at 17 weeks revealed that the superficial layer of peri-articular chondrocytes was enriched in cells with this surface phenotype. Isolation of cells with a similar immunophenotype from differentiating human PSCs revealed a population of CD166negBMPR1B+ putative pre-chondrocytes. Functional characterization confirmed these cells as cartilage-committed, chondrocyte progenitors. The identification of a specific molecular signature for primary cartilagecommitted progenitors may provide essential knowledge for the generation of purified, clinically relevant cartilage cells from PSCs. A total of 15 samples were analyzed. In the first comparison, there were 6 biological replicates for both the chondrogenic condensations and total limb cells. In the second comparison, three biological replicates of chondrocytes from the articular region were compared to the 6 replicates of the condensations.

2013-12-16 | E-GEOD-51812 | biostudies-arrayexpress

Identification of chondrocyte subsets during human development

2013-12-16 | GSE51812 | GEO

Identification of transcription factors potentially involved in the juvenile to adult phase transition in rough lemon (C. jambirhi Lush.)

2013-07-27 | GSE39798 | GEO

Identification of transcription factors potentially involved in the juvenile to adult phase transition in rough lemon (C. jambirhi Lush.)

Project description:The main objective of the present study was to identify citrus transcrition factors putatively involved in the juvenile to adult transition in citrus. A oligonucleotide microarray containing 1152 putative unigenes of citrus transcription factors was used. Rough lemon (C. jambirhi Lush.), were analyzed in two diferent developmental stages, junenile and adult. Four replicates for each sample category were generated and for each genotipe juvenile versus adult samples were compared . Comparative transcriptomic hybridization

2013-07-27 | E-GEOD-39798 | biostudies-arrayexpress

Identification of transcription factors potentially involved in the juvenile to adult phase transition in Pineapple sweet orange (C. sinensis (L.)

2013-07-27 | GSE39799 | GEO

Identification of transcription factors potentially involved in the juvenile to adult phase transition in Pineapple sweet orange (C. sinensis (L.)

Project description:The main objective of the present study was to identify citrus transcrition factors putatively involved in the juvenile to adult transition in citrus. A oligonucleotide microarray containing 1152 putative unigenes of citrus transcription factors was used. Pineapple sweet orange (C. sinensis (L.) was analyzed in two diferent developmental stages, junenile and adult. Four replicates for each sample category were generated and for each genotipe juvenile versus adult samples were compared . Comparative transcriptomic hybridization

2013-07-27 | E-GEOD-39799 | biostudies-arrayexpress

Sirt3 Promotes Chondrogenesis, Chondrocyte Mitochondrial Respiration and the Development of High-Fat Diet-Induced Osteoarthritis in Mice

Project description:Cartilage is characterized by mature chondrocyte metabolic heterogeneity in a zone-dependent manner. In this study, we used single cell mRNA sequencing (scRNA-seq) to evaluate the corresponding functional and metabolic phenotypic heterogeneity of in vitro cultured primary juvenile murine chondrocytes

2022-12-18 | GSE192668 | GEO

Lineage-Specific Differences and Inference of Regulatory Networks Governing Human Chondrocyte Development

Project description:In-vitro differentiation of chondrocyte populations recapitulate in-vivo behaviours, and reveal gene regulatory networks involved in chondrocyte development

2023-04-03 | GSE195688 | GEO

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