Project description:ChIP-seq profile of Rec8 binding sites arrested in metaphase I by depletion of the APC/C activator CDC20. The aim of this experiment is to identify Rec8 binding sites in metaphase I.
Project description:Proper progression through mitosis requires coordinated changes in RNA metabolism, but the specific contributions of individual RNA-binding proteins remain unclear. Heterogeneous nuclear ribonucleoprotein C (hnRNPC) is a ubiquitous RBP that normally binds uridine-rich sequences to influence pre-mRNA processing and stability. Here we investigated the role of hnRNPC in mitotic cells by profiling both global RNA abundance and hnRNPC–RNA interactions during metaphase arrest. To this end, we combined bulk RNA-seq with fluorescent crosslinking and immunoprecipitation (fCLIP) in proTAME-arrested HeLa S3 cells. This dataset enables the characterization of hnRNPC binding to mature RNAs during mitosis, the evaluation of biochemical fractionation (low-density vs high-density complexes), and the assessment of binding site resolution across distinct RNase digestion conditions. Together, the study provides a resource for exploring how hnRNPC contributes to RNA stability and gene expression control during cell division
Project description:Purpose: The goal is to determine the requirement for Chl4 in association of cohesin with the centromere and pericentromere boundaries