Project description:Tudor domain containing protein 9 (TDRD9) is a RNA helicase normally expressed in the germline. We have shown that TDRD9 is highly expressed in a subset of non-small cell lung carcinomas. Here we determine the transcriptomic changes of a TDRD9-positive lung tumor cells line (NCI-H1993) depleted of TDRD9.
Project description:Conventional in vitro and in vivo models often fail to accurately predict clinical outcomes due to their inability to capture the full complexity of the tumor microenvironment (TME). Existing 3D models also face difficulties in reproducing TME complexity and encounter various engineering limitations. In this study, 3D embedded bioprinting was used to construct a heterogeneous lung spheroid (HLS) model that integrates key stromal factors to more closely simulate the TME. RNA sequencing–based transcriptomic profiling revealed gene signatures related to extracellular matrix remodeling, immune suppression, and tumor progression, which showed strong resemblance to patient-derived lung tumor samples and supported the model’s biological relevance. In summary, this model reproduces essential characteristics of the lung TME and holds promise as a tool for assessing advanced therapies targeting complex solid tumors.
Project description:To characterize sotorasib resistance in lung adenocarcinomas (LUAD), we implanted pieces derived from a patient-derived KRAS-G12C positive xenograft (PDX) lung tumor model in immunocompromised mice