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Deletion of MtrA inhibits cellular development of Streptomyces coelicolor and alters expression of developmental regulatory genes

ABSTRACT: Deletion of MtrA inhibits cellular development of Streptomyces coelicolor and alters expression of developmental regulatory genes

PROVIDER: PRJNA412198 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6077060_1.fastq.gz	Fastqsanger.gz
	SRR6077060_2.fastq.gz	Fastqsanger.gz
	SRR6077062_1.fastq.gz	Fastqsanger.gz
	SRR6077062_2.fastq.gz	Fastqsanger.gz
	SRR6077064_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

Deletion of MtrA inhibits cellular development of Streptomyces coelicolor and alters expression of developmental regulatory genes

Project description:The developmental life cycle of Streptomyces species includes aerial hyphae formation and spore maturation, two distinct developmental processes that are controlled, respectively, by two families of developmental regulatory genes, bld and whi. In this study, we show that the response regulator MtrA (SCO3013) is critical for normal development of aerial hyphae in S. coelicolor and related species. ΔmtrA, a deletion mutant of the response regulator gene mtrA, exhibited the bald phenotype typical of bld mutants defective in aerial mycelium formation, with formation either much delayed or absent depending on the culture medium. Transcriptional analysis indicated that MtrA activates multiple genes involved in formation of aerial mycelium, including chp, rdl, and ram genes, as well as developmental regulatory genes of the bld and whi families. However, the major regulatory gene bldD showed enhanced expression in ΔmtrA, suggesting it is repressed by MtrA. EMSAs indicated that MtrA binds upstream of several genes with altered expression in ΔmtrA, including bldD and whiI, and sequences similar to the consensus binding sequence for MtrA of another actinomycete, Mycobacterium tuberculosis, were found in the bound sites. A loosely conserved recognition sequence containing two short, direct repeats was identified for MtrA of S. coelicolor and was validated using mutational analysis. MtrA homologues are widely distributed among Streptomyces species, and as with S. coelicolor, deletion of the mtrA homologues sve_2757 from S. venezuelae and sli_3357 from S. lividans resulted in conditional bald morphology. Our study suggests a critical and conserved role for MtrA in Streptomyces development.

2017-09-27 | GSE104263 | GEO

Deletion of MtrA Inhibits Cellular Development of Streptomyces coelicolor and Alters Expression of Developmental Regulatory Genes.

Project description:The developmental life cycle of Streptomyces species includes aerial hyphae formation and spore maturation, two distinct developmental processes that are controlled, respectively, by two families of developmental regulatory genes, bld and whi. In this study, we show that the response regulator MtrA (SCO3013) is critical for normal development of aerial hyphae in S. coelicolor and related species. ΔmtrA, a deletion mutant of the response regulator gene mtrA, exhibited the bald phenotype typical of bld mutants defective in aerial mycelium formation, with formation either much delayed or absent depending on the culture medium. Transcriptional analysis indicated that MtrA activates multiple genes involved in formation of aerial mycelium, including chp, rdl, and ram genes, as well as developmental regulatory genes of the bld and whi families. However, the major regulatory gene bldD showed enhanced expression in ΔmtrA, suggesting it is repressed by MtrA. electrophoretic mobility shift assays indicated that MtrA binds upstream of several genes with altered expression in ΔmtrA, including bldD and whiI, and sequences similar to the consensus binding sequence for MtrA of another actinomycete, Mycobacterium tuberculosis, were found in the bound sites. A loosely conserved recognition sequence containing two short, direct repeats was identified for MtrA of S. coelicolor and was validated using mutational analysis. MtrA homologs are widely distributed among Streptomyces species, and as with S. coelicolor, deletion of the mtrA homologs sve_2757 from S. venezuelae and sli_3357 from S. lividans resulted in conditional bald morphology. Our study suggests a critical and conserved role for MtrA in Streptomyces development.

| S-EPMC5650626 | biostudies-literature

Global effects of pathway-specific regulator CpkO and CpkN deletion in Streptomyces coelicolor A3(2)

Project description:Relative quantification of protein abundances of S. coelicolor A3(2) parent strain M145 and deletion mutants ∆cpkO and ∆cpkN. This study shows novel insights into the mechanism of coelimycin synthesis regulation and the network of interactions between cpk cluster regulatory proteins and the biosythetic genes of other secondary metabolite clusters.

2021-03-29 | PXD012672 | Pride

S coelicolor ∆argR vs S. coelicolor M145

Project description:S. coelicolor M145 and S. coelicolor ∆argR were grown in MG medium and samples from 3 biological replicates were taken at 32, 42, 49, 56 and 66 hours.

2015-08-19 | GSE58666 | GEO

RNA-seq of Streptomyces coelicolor hupA and hupS deletion strain

Project description:RNA-seq of Streptomyces coelicolor hupA and hupS deletion strain

| PRJEB72891 | ENA

Transcription profiling of Streptomyces coelicolor wild type and zur deletion strains

Project description:Description: In Streptomyces coelicolor the Zur repressor controls genes involved in zinc uptake and alternative ribosomal proteins that lack structural zinc. This experiment provides global gene expression patterns of M145 and S121 (zur deletion) grown in NMMP plus glucose medium to mid-log phase. The data reveal that Zur also controls genes involved in production of a non-ribosomally encoded siderophore-related peptide designated coelibactin.

2010-10-13 | E-MAXD-56 | biostudies-arrayexpress

Metabolic switching of the PII protein GlnK in Streptomyces coelicolor

Project description:GlnK is an important nitrogen sensor protein in Streptomyces coelicolor. Deletion of glnK results in a medium-dependent failure of aerial mycelium and spore formation and loss of antibiotic production. Thus, GlnK is not only a regulator of nitrogen metabolism but also of morphological differentiation and secondary metabolite production. Through a comparative transcriptomic approach between the S. coelicolor wild-type and a S. coelicolor glnK mutant strain, 142 genes were identified that are differentially regulated in both strains. Among these are genes of the ram and rag operon, which are involved in S. coelicolor morphogenesis, as well as, genes involved in gas vesicle biosynthesis and ectoine biosynthesis. Surprisingly, no relevant nitrogen genes were found to be differentially regulated, revealing that GlnK is not an important nitrogen sensor under the tested conditions.

2011-12-31 | GSE30570 | GEO

ANTIBIOTICS OVERPRODUCTION IN STREPTOMYCES COELICOLOR A3(2) MEDIATED BY PHOSPHOFRUCTOKINASE A2 DELETION

Project description:Gene expression analysis of S. coelicolor M145 and the delta_pfkA2 mutant. RNA samples were taken in the early exponential phase during growth in fermentors in defined mineral medium. Keywords: cell type, gene expression, genetic modification

2008-05-01 | GSE7686 | GEO

Streptomyces coelicolor

Project description:Time course of Streptomyces coelicolor: M145 Control vs. wblA deletion mutant

| PRJNA132889 | ENA

Time course of Streptomyces coelicolor: M145 Control vs. wblA deletion mutant

Project description:The basic experiment is a comparison of gene transcript levels of Streptomyces coelicolor M145 and its wblA deletion mutant at 6 timepoints (24, 36, 48, 60, 72 and 84 hours) encompassing vegetative growth, aerial growth, and sporulation on solid medium. The wblA mutant is morphologically defective, most of its aerial hyphae failing to show any sign of sporulation-directed attributes, and also overproduces some antibiotics. The microarray analysis was aimed to reveal the major transcriptional changes underpinning or associated with this pleiotropic phenotypic change. Using a fairly stringent cut-off, 291 genes were found to be affected, including developmental genes, antibiotic biosynthetic genes, and genes for primary metabolism. Some genes were over-expressed and others under-expressed in the mutant. Although the largest effects were mostly at timepoints corresponding to aerial growth and early sporulation, some genes were most strongly influenced at earlier timepoints.

2011-01-28 | GSE24396 | GEO

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