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CRP and FIS

ABSTRACT: CRP and FIS

PROVIDER: PRJNA412865 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR5099120_1.fastq.gz	Fastqsanger.gz
	SRR5099120_2.fastq.gz	Fastqsanger.gz
	SRR5099130_1.fastq.gz	Fastqsanger.gz
	SRR5099130_2.fastq.gz	Fastqsanger.gz
	SRR5099140_1.fastq.gz	Fastqsanger.gz

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ChIP-Seq experiment for the global regulators, CRP and Fis in early and mid exponential growth phases

Project description:We have performed ChIP-Seq experiment for the global regulators, CRP and Fis in early and mid exponential growth phases respectively in Escherichia coli K12 MG1655. The dataset contains the genome wide binding patterns of Fis and CRP in the wildtype and the mutant strains

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Crosstalk between global regulators CRP and FIS in E. coli.

Project description:Crosstalk between global regulators CRP and FIS in E. coli.

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Fis occupancy along the Salmonella enterica serovar Typhimurium SL1344 fis::3XFlag strain grown in different aeration regimes.

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Project description:Fis is a nucleoid-associated protein in E. coli that is abundant during early logarithmic growth in rich medium but is in short supply during stationary phase. Its role as a transcriptional regulator has been demonstrated for an increasing number of genes. In order to gain insight into the global effects of Fis on E. coli gene expression during different stages of growth in rich medium, DNA microarray analyses were conducted in fis and wild type strains during early log, mid log, late log, and stationary growth phases. We used microarrays to detail the global impact of Fis on gene expression in Escherichia coli Keywords: time course

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CRP and FIS

Project description:This SuperSeries is composed of the SubSeries listed below.

2017-10-03 | GSE104505 | GEO

Transcription profiling of E. coli covering four phases of growth in Fis KO and WT (Fis+) strains

Project description:This SuperSeries is composed of the following subset Series:; GSE7379: Fis KO strain; GSE7380: WT (Fis+) strain Experiment Overall Design: Refer to individual Series

2008-06-15 | E-GEOD-7398 | biostudies-arrayexpress

Fis occupancy along the Salmonella enterica serovar Typhimurium SL1344 fis::3XFlag strain grown in different aeration regimes.

2013-05-01 | E-GEOD-38661 | biostudies-arrayexpress

Network rewiring: physiological consequences of reciprocally exchanging the physical locations and growth-phase-dependent expression patterns of the Salmonella fis and dps genes. [ChIP-Seq]

Project description:The Fis nucleoid-associated protein controls the expression of a large and diverse regulon of genes in Gram-negative bacteria. Fis production is normally maximal in bacteria during the early exponential phase of batch culture growth, becoming almost undetectable by the onset of stationary phase. We tested the effect of rewiring the Fis regulatory network in Salmonella by moving the complete fis gene from its usual location near the origin of chromosomal replication to the position normally occupied by the dps gene in the Right macrodomain of the chromosome, creating the strain GX. In a parallel experiment, we tested the effect of placing the fis open reading frame under the control of the stationary-phase-activated dps promoter at the dps genetic location within Ter, creating the strain OX. ChIP-seq was used to measure global Fis protein binding and gene expression patterns. Strain GX showed few changes when compared with the wild type, although we did detect increased Fis binding at Ter, accompanied by reduced binding at Ori. Strain OX displayed a more pronounced version of this distorted Fis protein-binding pattern together with numerous alterations in the expression of genes in the Fis regulon. OX, but not GX, had a reduced ability to infect cultured mammalian cells, had undergone a reduction in competitive fitness and had reduced motility compared to the wild type. These findings illustrate the inherent robustness of the Fis regulatory network to rewiring based on gene repositioning alone and emphasise the importance of fis expression signals in phenotypic determination.

2020-07-06 | GSE152225 | GEO

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