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Short-read nascent RNA sequencing from S. pombe

ABSTRACT: Short-read nascent RNA sequencing from S. pombe

PROVIDER: PRJNA413497 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6144340.fastq.gz	Fastqsanger.gz
	SRR6144341.fastq.gz	Fastqsanger.gz
	SRR6144342.fastq.gz	Fastqsanger.gz
	SRR6144343.fastq.gz	Fastqsanger.gz
	SRR6144344.fastq.gz	Fastqsanger.gz

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Short-read nascent RNA sequencing from S. pombe

Project description:Short-read sequencing of nascent RNA from exponentially growing S. pombe cells was employed to quantify co-transcriptional splicing and expression levels. Nascent RNA was prepared from the yeast chromatin fraction (Carrillo Oesterreich, Preibisch, Neugebauer, Mol Cell 2010). Single-end Illumina sequencing data were generated from chromatin-associated, non-polyadenylated RNA (nascent RNA) and cytoplasmic mRNA for reference. In an experiment to test for splicing-associated gene expression changes cells were treated with 10mM Caffeine for 15minutes and nascent and mRNA-seq data were generated subsequently.

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Long-read nascent RNA sequencing from S. pombe

Project description:Long-read nascent RNA sequencing from S. pombe

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Project description:Long read SMRT cDNA sequencing of nascent RNA from exponentially growing S. cerevisiae and S. pombe cells was employed to obtain transcription elongation and splicing information from single transcripts. Nascent RNA was prepared from the yeast chromatin fraction (Carrillo Oesterreich, Preibisch, Neugebauer, Mol Cell 2010). The nascent 3’ end was labeled with a 3’ DNA adaptor through ligation. The adaptor sequence served as template for full-length reverse transcription and double-stranded cDNA was obtained in a PCR (gene-specific or transcriptome-wide). SMRT DNA sequencing libraries were prepared subsequently.

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Long-read sequencing of nascent RNA in S. pombe reveals coupling among RNA processing events

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